A stability-indicating assay by reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of pentobarbital sodium in oral formulations: a drug used for infant sedation in computed tomography (CT) or magnetic resonance imaging (MRI) scan. The chromatographic separation was achieved on a reversed-phase C18 column, using isocratic elution and a dete...

We studied the mechanism of barbiturate protection from focal cerebral infarction in cats by examining in detail edema formation 72 hours after acute, permanent occlusion of the left middle cerebral artery (LMCA). Neurological function, gas exchange, vital signs, and intracranial pressure (ICP) were observed during the post-occlusion period, and infarct size and cerebral edema were measured aft...

The Mongolian gerbil is known to develop delayed neuronal death in the hippocampus following brief forebrain ischemia (Brain Res 239: 57-69, 1982). The effect of pentobarbital on this slow process of neuronal damage was examined. Immediately following 5 min of bilateral carotid occlusion, pentobarbital (10, 20, or 40 mg/kg) was injected. The control animals received saline injection. Seven days...

Rosa damascena has been found to act on central nervous system including the brain. Several studies confirm that R. damascena inhibits the activity of the hypothalamus and pituitary systems in rat and can suppress the central nervous system. In traditional medicine hypnotic effect of Rose is also suggested. In this study, the hypnotic effect of the ethanol crude extract of R. damascena and its ...

We examined the effect of a range of pentobarbital concentrations on 0.5 mM g-aminobutyric acid (GABA)-activated channels (10 6 1 pS) in inside-out or outside-out patches from rat cultured hippocampal neurons. The conductance increased from 12 6 4 to 62 6 9 pS as the pentobarbital concentration was raised from 10 to 500 mM and the data could be fitted by a Hill-type equation. At 100 mM pentobar...

DURING measurements of pressure in the small veins of a dog's hindleg, it was observed that fluctuations in pressure, associated with respiration, varied considerably from animal to animal. In some instances, venous pressure fell during the inspiratory phase; in other instances, venous pressure rose. In addition, spontaneous pressure changes, seemingly independent of respiratory movements, were...

BACKGROUND The Canadian Council on Animal Care and American Veterinary Medical Association classify intraperitoneal (IP) pentobarbital as an acceptable euthanasia method in rats. However, national guidelines do not exist for a recommended dose or volume and IP euthanasia has been described as unreliable, with misinjections leading to variable success in ensuring a timely death. The aims of this...