In a study of genetic lesions respo"sible for amino acid requirements in multiple auxotrophic lactic aeid bacteria, a systematic attempt was made to isolate mutants that could synthesize each of the amino acids required by the parental strains of Lactocoecus lactis, Enterocoecus flaecium, and Pediococcus acidilactiei. After treatment with appropriate mutagens or, in some cases, spontaneously, s...

peptidoglycan layer of the bacterial cell wall,3) and accordingly can be a target fbr development of new antibiotics. We purified the enzyme from Pediococcus pentosaeeus4) and cloned its gene into Eycherichia colii'2} to construct an overproducer,S) We here report that L-serine O-sulfate, an analog of L-glutamate, serves as a suicide substrate to inactivate the enzyme, This is thc first example

DNA sequences covering 36% of the mle gene that encodes the malolactic enzyme were determined for 13 strains of lactic acid bacteria, representing Pediococcus, Leuconostoc, Lactobacillus and Oenococcus genera. The sequences were aligned with the corresponding region of mleS in Lactococcus lactis. The phylogenetic distance matrix tree of all mle sequences was compared with the 16S rRNA phylogene...

Lactobacillus plantarum 423 produces a small heat-stable antimicrobial protein designated plantaricin 423. This protein is bactericidal for many Gram-positive foodborne pathogens and spoilage bacteria, including Listeria spp., Staphylococcus spp., Pediococcus spp., Lactobacillus spp., etc. The DNA sequence of the plantaricin 423-encoding region on plasmid pPLA4 revealed a four open reading fram...

Listeria monocytogenes is a foodborne pathogen associated with fermented products. The aim of this study was to characterize anti-listerial bacteriocin produced by lactic acid bacteria (LAB) isolated from Cambodian fermented foods. Four LAB isolates of confirmed bacteriocin production were isolated from fermented fish and shrimp (pha ak trey, pha ak kampus). These isolates demonstrated antimicr...