Co-expression of enzymes allow to produce multiple in a single host, representing cost-effective alternative biocatalytic processes which can be used for pectin bioconversion. Pectin-rich biomass is an abundant by-product from the fruit and sugar industries that usually disposed landfill or sold as low value feedstock. The aim this work was co-express thermophilic methyl esterase (PME) exo-poly...

Pectic oligomers induced a rapid, transient increase in ethylene biosynthesis when added to pear cells in suspension culture. The rate of ethylene biosynthesis increased within 30 to 40 minutes after oligomer addition, reached a maximum between 90 and 120 minutes after addition, and then decreased to basal rates of synthesis. Both the rapid increase and decrease in biosynthesis appear to be pre...

Developing sunflower (Helianthus annuus L.) seeds were analyzed for their fresh and dry weights, water content and wall components viz. low and high molecular weight xyloglucan and pectic polysaccharides. Seeds from two rows (6th and 12th) differing in their final dry weight and position on the capitulum were selected for the investigation. The dry weight of seed from 6th row was 69 mg while th...

Polygalacturonic acid (PGA) synthase successively transfers galacturonic acid to oligogalacturonic acid by an alpha1,4-linkage to synthesize PGA, the backbone of plant pectic homogalacturonan. PGA synthase has not been purified to date due to its instability in vitro. In this study, we found stable conditions in vitro and separated a minimum active component of the enzymes from pea and azuki be...

Vatanparast M., Hosseininaveh V., Ghadamyari M., Minoo Sajjadian S. (2014): Plant cell wall degrading enzymes, pectinase and cellulase, in the digestive system of the red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae). Plant Protect. Sci., 50: 190–198. In digestion, the red palm weevil, Rhynchophorus ferrugineus, has been adapted to overcome the plant cell wall barrier, spec...