except at corners and pit fields. In contrast, pectic side-chains Six monoclonal antibodies (mAbs) were used to map the were not detectable in the middle lamella of all parenchymal distribution of pectic epitopes in the cell walls of potato cell walls, except in the cortex where mAb LM6 (arabinan (Solanum tuberosum L. cvs Kardal and Karnico) tuber tissue epitope) labelled the entire wall. The g...

The cell-wall polysaccharides of Arabidopsis thaliana leaves have been isolated, purified, and characterized. The primary cell walls of all higher plants that have been studied contain cellulose, the three pectic polysaccharides homogalacturonan, rhamnogalacturonan I and rhamnogalacturonan II, the two hemicelluloses xyloglucan and glucuronoarabinoxylan, and structural glycoproteins. The cell wa...

AIMS To compare the in vitro fermentation properties of pectins and oligosaccharides derived from them in pure and mixed faecal cultures. METHODS AND RESULTS Specific growth rates of selected bacterial genera were calculated in pure culture. Bifidobacterium angulatum, B. infantis and B. adolescentis had higher growth rates on pectic oligosaccharides (POS I) derived from high methylated pectin...

Cell walls of grasses have two major polysaccharides that contain uronic acids, the hemicellulosic glucuronoarabinoxylans and the galactosyluronic acid-rich pectins. A technique whereby esterified uronic acid carboxyl groups are reduced selectively to yield their respective 6,6-dideuterio neutral sugars was used to determine the extent of esterification and changes in esterification of these tw...

[U-(14)C]Sucrose, myo-[U-(14)C]inositol, [6-(14)C]- and [U-(14)C]glucuronate, UDP-[U-(14)C]glucuronate, [U-(14)C]gluconate, and l-[1-(14)C]ascorbic acid were fed into grape berries, Vitis labrusca L. cv. Delaware, at intervals throughout the ripening process and incorporation of (14)C into several metabolites was studied.[U-(14)C]Sucrose was the most effective precursor of cellulose in young gr...

The alcohol-insoluble fraction from the water extract of tamarind seed meal, constituting 60 to 65 per cent of the husked kernel, has been described as a rich source of pectin (l-3). Although it forms a firm jelly in the presence of appropriate amounts of sugar and acid (1,4) and has been suggested for commercial use as a substitute for pectin (5), it has been shown in preliminary communication...

The pectic enzymes produced in vitro by 8 isolates (5 Highly virulent and 3 Weakly virulent) of Fusarium oxysporum , were detected by spectrophotometry, and characterized by polyacrylamide gel electrophoresis with substrate-containing gels (zymogram). Analysis of the polygalacturonase (PG) isozyme banding patterns (zymogram) identified two distinct phenotypes among the isolates from chickpea (C...