Identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomic changes accompanying development. Ideally expression of these genes should be independent of the morphogenetic process or environmental condition.We report here the validation of internal control gene i.e.TPS (trehalose 6-phosphate-synthase) in cotton (Gossypium spp), using TaqMan sy...

Fifty-five isolates of Escherichia coli from septicaemic neonatal foals were used to validate five real-time pcr assays targeting different known virulence factor genes: curli fibre (csgD), ferric hydroxamate uptake (fhuA), type 1A pilin (fimA), aerobactin (lutA) and yersiniabactin (fyuA). A pcr assay targeting a universal sequence of the bacterial 16S rrna gene served as quality control. The p...

Background: There is a conserved portion in the 16S rRNA gene of bacteria which can be amplified by the universal PCR method. This fragment is 996 bp in length. In this method, only one set of universal primers is used for the amplification of the conserved region of the 16S rRNA gene, in common bacterial pathogens. Therefore, using the universal PCR method, these bacteria are detectable on...

background: among the most important parasitic disease, causing diarrhea, gi­ardia lamblia is noteworthy. nowadays detection methods for these parasites in­clude parasitological methods such as microscopic examination. the sensitivity of these methods relies on the expertise and experience of examiners. in contrast, molecular methods such as pcr are less dependent on the expertise of the exam­i...

brucella melitensis and brucella abortus are of the most important causes of brucellosis, an infectious disease which is transmitted either directly or indirectly including consuming unpasteurized dairy products. both strains are considered endemic in iran. common diagnostic methods such as bacteriologic cultures are difficult and time consuming regarding the bacteria. the aim of this study was...

breast cancer is the most important and prevalent cancer in women that can be managed if detected in the early stages. recently, there has been much focus on the use of epigenetic changes such as dna methylation in early detection of cancer. as recent research has put forth dok7 cpg island methylation as an epimarker, in the present study, using a quantification method, a cohort of iranian spor...

Background: Staphylococcal food poisoning (SFP) is one of the most common food-borne diseases worldwide resulting from contamination by preformed S. aureus enterotoxins and milk considered to be a major source SFP. This study aimed at PCR based detection its enterotoxigenic genes in raw products. Methods: A total 300 samples products were collected randomly Aizawl district Mizoram subjected phe...

A real-time reverse transcriptase PCR (RRT-PCR) assay based on the avian influenza virus matrix gene was developed for the rapid detection of type A influenza virus. Additionally, H5 and H7 hemagglutinin subtype-specific probe sets were developed based on North American avian influenza virus sequences. The RRT-PCR assay utilizes a one-step RT-PCR protocol and fluorogenic hydrolysis type probes....

1, 4, 5, 6-Tetrahydro-2-methyl-4-pyrimidine carboxylic acid (ectoine) is an excellent osmoprotectant. Ectoine and hydroxyl ectoines are of great significance to the biotechnology industry, thus the detection and isolation of ectoine producing bacteria is of great importance. Hence, this study involved the detection of the ectC gene (encoding ectoin synthase enzyme) using polymerase chain reacti...