نتایج جستجو برای: pcr cloning
تعداد نتایج: 235102 فیلتر نتایج به سال:
Background and Aim: Leishmania major p4 gene is normally expressed during amastigote form of the parasite and can be a good candidate for producing an effective vaccine. The aim of this study is to clone p4 gene in a suitable vector for further vaccine production studies .Materials and Methods: This is an experimental study. Leishmania promastigote was grown in N.N.N. medium and later in th...
Background: Recent researches have demonstrated that human T-cell immunoglobulin mucin 1 (TIM-1) glycoprotein plays important roles in regulation of autoimmune and allergic diseases, as well as in tumor immunity and response to viral infections. Therefore,targeting TIM-1 could be a potential therapeutic approach against such diseases. Objectives: In this study, we aimed to express TIM-1 protein...
A major application of polymerase chain reaction (PCR) is the cloning of DNA fragments with known sequences, e.g., full-length cDNAs and genomic fragments. Although the introduction of the long PCR procedure (2) has facilitated this PCR-based cloning, its usefulness is limited by the high rates of faulty nucleotide incorporation that is seen in all heat-stable DNA polymerases (6). Although seve...
BACKGROUND Expression of higher eukaryotic genes as soluble, stable recombinant proteins is still a bottleneck step in biochemical and structural studies of novel proteins today. Correct identification of stable domains/fragments within the open reading frame (ORF), combined with proper cloning strategies, can greatly enhance the success rate when higher eukaryotic proteins are expressed as the...
Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.
Although the polymerase chain reaction (1,2) (PCR) can be used to produce a large amount of a specific DNA from a complex source, cloning the PCR products has not proven to be straightforward. Restriction endonuclease sites are often incorporated into the oligonucleotide primers used for amplification, so that cleavage of the product will create sticky ends that can theoretically be ligated to ...
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