نتایج جستجو برای: pcr based marker
تعداد نتایج: 3177678 فیلتر نتایج به سال:
aim : we aimed to develop a multiplex pcr assay for specific detection of epec and ehec pathotypes based on specific marker genes. background : about 2.5 million infant’s morbidity in developing countries occurs by e.coli pathotypes because of diarrhea and intestinal diseases. the traditional phenotypic methods are time consuming and sometimes detection and differentiation of the pathotypes are...
AIMS To develop a multiplex PCR targeting the gyrB and pntA genes for Vibrio species differentiation. METHODS AND RESULTS Four pairs of primers targeting gyrB gene of Vibrios at genus level and pntA gene of Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus were designed. This PCR method precisely identified 250 Vibrio species and demonstrated sensitivity in the range of 4 x 10(4) CF...
A PCR-method for fast production of disruption cassettes is introduced, that allows the addition of long flanking homology regions of several hundred base pairs (LFH-PCR) to a marker module. Such a disruption cassette was made by linking two PCR fragments produced from genomic DNA to kanMX6, a modification of dominant resistance marker making S. cerevisiae resistant to geneticin (G418). In a fi...
Short tandem repeat (STR) typing has become the workhorse of modern forensic DNA analysis. The most common form of STR typing uses laser-induced fluorescence detection of dye-labeled polymerase chain reaction (PCR) products following capillary electrophoresis (CE) size-based separation. This unit describes the techniques and marker systems most widely used around the world in constructing crimi...
BACKGROUND The proliferation marker Ki67 has been suggested as a promising cancer biomarker. As Ki67 needs an exact quantification, this marker is a prototype of a new generation of tissue-based biomarkers. In this study, we have systematically evaluated different cut points for Ki67 using three different clinical end points in a large neoadjuvant study cohort. PATIENTS AND METHODS We have ev...
Colorectal cancer (CRC) is considered one of the leading types in world. CD133, as a stem cell marker, has pivotal role development drug resistance, migration, and stemness properties CRC cells. This study was designed to check combined effect CD133 siRNA Oxaliplatin on proliferation, apoptosis, cells HT-29 line. MTT assay performed define viability cells, it showed that combination could reduc...
To aid in identifying key predators of Proconiini sharpshooter species present in California, we developed and tested molecular diagnostic markers for the glassy-winged sharpshooter, Homalodisca coagulata (Say), and smoke-tree sharpshooter, Homalodisca liturata (Ball) (Homoptera: Cicadellidae). Two different types of markers were compared, those targeting single-copy sequence characterized ampl...
Figure 1. Diagram of a split-marker gene replacement strategy using fusion-PCR (A) FPCR step 1 amplification of flanking regions and partial marker segments. Dotted tails indicate the overlap sequences on selected primers which allow fusion in step 2. (B) FPCR step 2 flanking regions are fused to the marker segments at the overlap sights, forming two constructs. The fusion products are then amp...
Abstract With the globalization of seed trade and transgenic variety development, application molecular technologies for quality gained more significance in both internal international markets. Besides germination, genetic purity health are two most important parameters that determine planting value a lot. Compared to conventional methods testing, marker efficient analysis as these accurate, ro...
background : hepatitis includes a wide range of clinical and pathological conditions. the beta-2 microglobulin (ãÿ2m), as part of thehla complex, is responsible for transmission of viral antigens on the surface of liver cells. the purpose of this study is to determine the concentration of salivary ãÿ2m as a marker of viral proliferation in subjects who are hbsag + , hbv dna pcr + compared with ...
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