Systemic interleukin 2 (IL-2) and IL-2-activated lymphocytes have induced tumor regression in some cancer patients. The IL-2-activated cells have usually been generated by obtaining peripheral blood mononuclear cells (PBMC) from cancer patients shortly after systemic IL-2 therapy and culturing them with IL-2 in vitro. In an effort to augment the ex vivo generation of such cells preactivated in ...

Studies were undertaken to determine whether leukemia and lymphoma cells would be lysed by autologous and allogeneic lymphokine-activated killer (LAK) cells. Peripheral blood mononuclear cells (PBMC) from patients and normal donors were cultured for five days, 2 weeks, and 4 weeks with medium containing 2,500 units of recombinant interleukin 2 (IL-2) per mL, and their cytotoxicity was assayed b...

BACKGROUND Reduced innate immunity responses as well as reduced T regulatory activities characterise bronchial asthma. OBJECTIVES In this study the effect of budesonide on the expression of TLR4 and TLR2 in T regulatory lymphocyte sub-population was assessed. METHODS TLR4 and TLR2 expression in total peripheral blood mononuclear cells (PBMC), in CD4+/CD25+ and in CD4+/CD25- was evaluated, b...

Abstract Background Recent studies have shown that immunotherapies, including peptide vaccines, remain promising strategies for patients with lower grade glioma (LGG); however new biomarkers need to be developed identify who may benefit from therapy. We aimed investigate the feasibility of liquid biopsy-based gene signatures in predicting prognosis LGG patients, as well benefits immunotherapy. ...

Figure 1. Detection of Borna disease virus (BDV)–specific nucleic acid in peripheral blood mononuclear cells (PBMC) of a man with chronic fatigue syndrome (CFS), by nested and seminested polymerase chain reaction assays. Lanes: A, BDV-negative PBMC; B, BDV-specific amplicates from PBMC of patient with CFS; M, size marker (100-bp DNA ladder). Demonstration of Borna Disease Virus Nucleic Acid in ...

Increased plasma NEFA concentrations and compromised immune responses are associated with increased disease susceptibility during farrowing and lactation. Increased plasma NEFA concentrations cause changes in the fatty acid (FA) content of plasma lipid fractions and peripheral blood mononuclear cells (PBMC) that could modify inflammatory responses. The goals of this study were to describe chang...

In multiple myeloma (MM), the VDJ rearrangement of the immunoglobulin heavy chain expressed by MM plasma cells provides a unique clonotypic marker. Although clonotypic MM cells have been found in the circulation, their number has been controversial. Our objective was to provide direct evidence, using single-cell assays, for the frequency of clonotypic cells in blood of 18 MM patients, and to co...

The cytokine production patterns of human peripheral blood mononuclear cells (PBMC) in response to Salmonella typhi flagella (STF) were examined in culture supernatants of PBMC stimulated with STF. Consistent with previous findings in volunteers vaccinated with aroC aroD deletion mutants of S. typhi, PBMC from volunteers immunized with the licensed live Ty21a S. typhi vaccine secreted gamma int...

The inhibition of unspecific adhesion of human white blood cells is a prerequisite for applications requiring the control of defined surface interactions. In this study, a passivation agent based on polyethylene glycol (PEG) for glass surfaces was investigated for the use with human peripheral blood mononuclear cells (PBMC). The grafting of 2000 g/mol methoxy-terminated PEG-urea-triethoxysilane...

A growing number of autoimmune diseases, allergies, and atopic conditions in recent years are calling for the development innovative therapies, such as those using stem cells with immunoregulatory activity. Because few often conflicting results scientific literature, it is still uncertain whether mesenchymal (MSC) could be a useful therapeutic strategy targeting B lymphocytes helpful treating a...