Embryos of the brine shrimp, Artemia salina, were used in a study of polypeptide chain initiation in an in vitro system from a eukaryote. A protein, isolated from the high-speed supernatant, has been highly purified and shown to have properties that suggest it is the eukaryotic equivalent of the Escherichia coli initiation factor F(2): It promotes the AUG-dependent binding of fMet-tRNA (E. coli...

Many different proteins associate with microtubules, influencing their spatial organization and function. These include proteins of a structural nature, which link microtubules to one another or to other cellular organelles and which may stimulate tubulin assembly. The second group, the so-called dynamic microtubuleassociated proteins, move subcellular components along microtubules through nucl...

Survival rate (SR) and growth rate (GR) were tested with various feed sources to identify an appropriate feed to improve the productivity in the early life stage of the rock worm Marphysa sanguinea (Montagu, 1813) (Eunicidae: Polychaeta). In addition, feed supply rates were also examined. Three experiments were performed to identify the appropriate feed for the juvenile stages of M. sanguinea. ...

Ann. Entomol. Soc. Am. 76: 400-402 (1983) ABSTRACT No males were found among 559 specimens of Pityophthorus puberulus (LeConte) from several localities. No sperm was found in the spermatheca of 10 females taken from recently established galleries containing eggs and young larvae. This is the third record of thelytoky in a scolytid. The known distribution and environment of parthenogenetic popul...

The growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear. In the following study we compared the effects of such diets on redox regulation pathways during development of Atlantic cod (Gadus morhua) larvae. Cod larvae were fed a control diet of copepods or the typical rotifer/Artemia diet commonly u...

Ž . Ž . Viable heterotrophic bacteria VHB -cultivated on ZoBell media and TCBS media TCBSB were enumerated from cysts that were hatched under laboratory sterile conditions as well as in a commercial hatchery without sterile conditions. Since the focus of this work was to study the TCBSB, 10 to 30 colonies growing on TCBS were isolated randomly from each sample. Results from 26 samples of differ...

Details of the life cycle of Aneugmenus merida Smith (Hymenoptera: Tenthredinidae) reared under laboratory conditions are reported. Females may produce parthenogenetic or non-parthenogenetic eggs. After eclosion, males of A. merida go through five larval instars, pre-pupa, pupa and imago. The complete life cycle lasts approximately 44 days. Larvae feed on bracken [Pteridium caudatum (L.) and Pt...

Nitric oxide (NO) is identified as a signaling molecule involved in many cellular or physiological functions including meiotic maturation and parthenogenetic activation of mammalian oocytes. We observed that nitric oxide donor SNAP was potent to induce parthenogenetic activation in Xenopus eggs. NO-scavenger CPTIO impaired the effects of SNAP, providing evidence for the effects of the latter to...

Parthenogenetically activated BCF1 and fertilized BALB/c embryos were aggregated to form chimaeras. The fate of the parthenogenetic component was followed in the conceptus during the second half of gestation. The results indicate an early strong selection against parthenogenetic cells in the extra-embryonal part, which is presumably complete by term, and a weaker selective process in the embryo...

In certain extraembryonic tissues of normal female mouse conceptuses, X-chromosome-dosage compensation is achieved by preferential inactivation of the paternally derived X. Diploid parthenogenones have two maternally derived X chromosomes, hence this mechanism cannot operate. To examine whether this contributes to the inviability of parthenogenones, XO and XX parthenogenetic eggs were construct...