Trypsin catalyses the hydrolysis of suitable derivatives of basic amino acids including peptides, amides and esters. Esters are the most sensitive substrates, and it was decided to use them in the present study, which had as one of its aims the elucidation of the relationship between the length of the side chain and susceptibility to hydrolysis by trypsin. Previous investigations had shown that...

Ogata, M., Takatsuka, Y., and Tomokuni, K. (1971). Brit. J. industr. Med., 28, 382-385. Excretion of hippuric acid and mor p-methylhippuric acid in the urine of persons exposed to vapours of toluene and mor p-xylene in an exposure chamber and in workshops, with specific reference to repeated exposures. Four male volunteers were exposed to 200 p.p.m. of toluene for five one-hour periods separate...

Superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), and the comet assay (SCGE) were used as biomarkers to evaluate the oxidative stress and genotoxicity of toluene, ethylbenzene and xylene in earthworms (Eisenia fetida). The results indicated that the exposure of the three pollutants caused a stress response of the three enzymes, an approximate bell-shaped change (a tendency ...

polypyrrole is one of the most useful conductive polymers, but green synthesis of this polymer has been restricted until now. the objective of the present paper is green synthesis of nano polypyrrole (ppy) by oxidative polymerization using urea-hydrogen peroxide. it was found that conductivity and morphology of polypyrrole are highly dependent on the reaction conditions such as the molar ratio ...

Multi-Walled Carbon Nanotubes (MWCNTs) surfaces were serially modified by the annealing treatment under Helium flow at 1000oC, the nitric acid treatment and again the annealing treatment under same conditions and their maximum adsorption capacities for benzene and toluene were measured and analyzed. The unmodified and modified MWCNTs werecharacterized by Fourier Tr...

HAVE BEEN USING the method of Pearson, Stern, and McGavack (1) for the determination of cholesterol for some time. It has proved to be a convenient and accurate procedure and the reaction had always gone smoothly. One day recently, however, the tubes were set up for the determination as usual, using a freshly prepared solution of p-toluenesulfonic acid. Solutions made from the same bottle of so...

Liquid chromatography/electrospray ionization/mass spectrometry (LC/ESI/MS) was used to determine intact phospholipid profiles for five reference pseudomonad strains harboring different (aerobic) toluene catabolic pathways: Pseudomonas putida mt-2, Pseudomonas putida F1, Burkholderia cepacia G4, Burkholderia pickettii PKO1, and Pseudomonas mendocina KR1. These five strains contained a predomina...

The preparation and full characterization of the 4-(nitrophenyl)phenoxyl radical, 2,6-di-(t)butyl-4-(4'-nitrophenyl) phenoxyl radical ((t)Bu2NPArO(•)) is described. This is a rare example of an isolable and crystallographically characterized phenoxyl radical and is the only example in which the parent phenol is also crystallographically well-defined. Analysis of EPR spectra indicates some spin ...