UNLABELLED The O-specific antigen (OSA) in Pseudomonas aeruginosa lipopolysaccharide is highly varied by sugar identity, side chains, and bond between O-repeats. These differences classified P. aeruginosa into 20 distinct serotypes. In the past few decades, O12 has emerged as the predominant serotype in clinical settings and outbreaks. These serotype O12 isolates exhibit high levels of resistan...

Pseudomonas aeruginosa is a major opportunistic pathogen in hospital-acquired infections and exhibits increasing antibiotic resistance. A rapid and sensitive molecular method for its detection in clinical samples is needed to guide therapeutic treatment and to control P. aeruginosa outbreaks. In this study, we established a polymerase spiral reaction (PSR) method for rapid detection of P. aerug...

We demonstrated that a 2% suspension of Pseudomonas aeruginosa alginate completely blocked the diffusion of gentamicin and tobramycin, but not that of carbenicillin, illustrating how alginate production can help protect P. aeruginosa growing within alginate microcolonies in patients with cystic fibrosis (CF) from the effects of aminoglycosides. This aminoglycoside diffusion barrier was degraded...

The production of β-lactamases is the most important mechanism of Gram-negative rod resistance to β-lactams. Resistance to ceftazidime and cefepime in clinical isolates of Enterobacteriaceae (especially ESβL-positive E. coli and K. pneumoniae) and P. aeruginosa is life-threatening. However, all strains of the above mentioned species possess chromosomally encoded RND efflux pump systems in addit...

In this study, among the 21 diesel-degrading bacteria that were isolated from an oil-polluted area in Fars (Iran), 6 bacterial strains were tested for their capability to metabolize and grow on diesel oil by degrading its hydrocarbons content. The biochemical characteristics and 16S rRNA sequence analysis of diesel-degrading bacteria showed that these strains were related to the genus Pseudomon...

The aim of this study was to evaluate the actiion of Clostridium perfringens neuraminidase on the adherence of 28 strains of Pseudomonas aeruginosa which were isolated from humans, different animals and environment to human buccal epithelial cells (BECs). Two reference strainns--NCTC 6749 and ATCC 27853 were also examined. Incubation of cells with the enzyme significantly increased bacterial ad...

Collagenase enzyme activity of 38 Pseudomonas aeruginosa strains and 38 strains of Escherichia coli from various pathological sources was measured by a simple method. This method uses plates with collagen gel. The rate of gel lysis is proportional to the collagenase concentration. The method is simple and requires no special materials or equipment. From the 38 P. aeruginosa strains, 34 were col...

The purpose of this study was to type the Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates recovered from cystic fibrosis (CF) patients by random amplified polymorphic DNA (RAPD)-PCR and to determine the antibiotic susceptibility of these strains. P. aeruginosa (n=49), and S. maltophilia (n=11) isolates which had been recovered from 16 and 8 patients, respectively, during a 1-ye...

A distinguishing feature of Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients is their mucoid, exopolysaccharide alginate-overproducing phenotype. One mechanism of conversion to mucoidy is based on mutations in the algU mucABCD cluster, encoding the stress sigma factor AlgU and its regulators. However, conversion to mucoidy in laboratory strains can be achieved via mutations in...