The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...

A single-step multiplex PCR (here referred to as a duplex PCR) has been developed for simultaneous detection and diagnosis of Fasciola hepatica and F. gigantica. These species overlap in distribution in many countries of North and East Africa and Central and Southeast Asia and are similar in egg morphology, making identification from fecal samples difficult. Based on a comparative alignment of ...

stevens-johnson syndrome (sjs) and toxic epidermal necrolysis (ten) are severe adverse cutaneous reactions to drugs. we describe the case of a 19 year old patient with sjs/ten overlap syndrome, who developed severe interstitial pneumonia after she had received antiepileptic drugs. a cytomegalovirus infection was diagnosed by real time polymerase chain reaction (rt-pcr) detection on bronchoalveo...

Abstract As an important class of aggregation operators, the notion overlap functions was first presented in 2009 order to be considered for applications image processing context. Later, many other researches arised bringing some variations those different purposes. Here, our main goal is defining on lattices and discuss how a weakned version it, named quasi-overlaps, works when continuity elim...

In this paper, we propose a novel ICP variant that uses a histogram in conjunction with multiple closest points to detect the overlap area between range images geing registered. Tentative correspondences sharing similar distances are normally all within, or all outside, the overlap area. Thus, the overlap area can be detected in a bin by bin batch manner using a histogram. Using multiple closes...

The performance of a novel thermal cycler has been characterized in a 30-cycle PCR. The device consists of a microcapillary equipped with bidirectional pressure-driven flow and in situ optical position sensors. A 1-microL droplet of reaction mixture moves between three heat zones in a 1-mm i.d., oil-filled capillary using a multi-element scattered light detector and active feedback. The design ...

The Additive Schwarz Method with Harmonic Extension (ASH) was introduced by Cai and Sarkis (1999) as an efficient variant of the additive Schwarz method that converges faster and requires less communication. We show how ASH, which is defined at the matrix level, can be reformulated as an iteration that bears a close resemblance to the parallel Schwarz method at the continuous level, provided th...

An extension of the modal overlap factor is introduced that includes ensemble variations and frequency averaging of plates and acoustic spaces. Uniformly and normally distributed variations of geometric and material parameters are considered. Using this modal overlap factor, approximate formulas are derived to identify the limit of predictability for the combined case of damping, ensemble varia...

We established new methods for cloning cDNA ends that start with reverse transcription (RT) and soon proceed with the synthesis of the second cDNA strand, avoiding manipulations of fragile RNA. Our 3'-end cloning method does not involve poly-dT primers and polymerase chain reactions (PCR), is low in efficiency but high in fidelity and can clone those RNAs without a poly-A tail. We also establis...