Abstract Background TERMINAL FLOWER 1 ( TFL1 ) belongs to the phosphatidylethanolamine-binding protein (PEBP) family, which is involved in inflorescence meristem development and represses flowering several plant species. In present study, four genes were cloned from mango Mangifera indica L.) variety ‘SiJiMi’ named MiTFL1-1 , MiTFL1-2 MiTFL1-3 MiTFL1-4 . Results Sequence analysis showed that en...

The expression of microRNA 198 which is localized in human chromosome 13q13.3 downregulated many malignant tumors. purpose this study to investigate the regulatory effects on non-small cell lung cancer and associated mechanism. levels lines tumor tissues were determined using reverse transcription polymerase chain reaction method. Overexpression adenocarcinomic alveolar basal epithelial cells, ...

Transaldolase, the key enzyme of pentose-phosphate pathway, plays an important role in plant growth and defense. Seed germination is a factor that influences cultivation Aquilaria sinensis, source agarwood, which widely used as traditional medicine, perfume incenses. However, little known about function transaldolase abscisic acid (ABA) signaling-mediated seed germination. In present study, ful...

The domestication gene Q is largely responsible for the widespread cultivation of wheat because it confers multiple traits. However, underlying molecular mechanisms how regulates these traits remain unclear. In this study, we identify a Q-interacting protein TaLAX1, basic helix–loop–helix transcription factor, through yeast two-hybrid assays. Using biochemical and genetic approaches, explore ro...

Abstract Background CSN5, a member of Cop9 signalosome, is essential for protein neddylation. It has been supposed to serve as an oncogene in some cancers. However, the role CSN5 not investigated cervical cancer yet. Methods Data from TCGA cohorts and GEO dataset was analyzed examine expression profile clinical relevance The on cell proliferation lines, Siha Hela, through knockdown via CRISPR–C...

Abstract Circular RNAs (circRNAs) play important roles in cancer tumorigenesis and progression, representing prognostic biomarkers therapeutic targets. In this case, we demonstrated the role of circ-NOLC1 epithelial ovarian (EOC). Our results have shown that Circ-NOLC1 expression was higher EOC tissues than normal tissues, positively associated with FIGO stage, differentiation. Among cell lines...

Objective: To explore the role of miR-448 in regulating MAGEA6/AMPK signaling pathway biological study hepatocellular carcinoma (HCC) tumor stem cells. Methods: Using database, related expression chips were obtained and regulatory mirnas candidate genes predicted, predicted results analyzed. The effects MAGEA6 on pellet formation rate clone cells detected by immunofluorescence identification ce...

Purpose: To investigate the biological functions of miR-423-5p in a mouse model with lipopolysaccharide (LPS)-induced acute liver injury.Methods: Sixty male C57BL/6 mice were randomized into control group (n = 20), LPS 20) and + mimic 20). The levels pro-inflammatory cytokines apoptosisassociated proteins tissues determined. Finally, survival was recorded within 7 days after injection LPS.Resul...

Background. The cortactin (CTTN) gene has been found, by transcriptomic profiling, to be overexpressed in pediatric osteosarcoma. The location of CTTN at 11q13 and the role of cortactin in cytoskeleton restructuring make CTTN of interest as a potential biomarker for osteosarcoma. Materials and methods. Osteoblasts were isolated from 20 high-grade osteosarcomas before chemotherapy, and paired wi...

The Rhodobacter sphaeroides system has been used to express membrane proteins. However, its low yield has substantially limited its application. In order to promote the protein expression capability of this system, the pucC gene, which plays a crucial role in assembling the R. sphaeroides light-harvesting 2 complex (LH2), was overexpressed. To build a pucC overexpression strain, a pucC overexpr...