We present a strategy to identify and map large numbers of transposon insertions in the genome of Caenorhabditis elegans. Our approach makes use of the mutator strain mut-7, which has germline-transposition activity of the Tc1/mariner family of transposons, a display protocol to detect new transposon insertions, and the availability of the genomic sequence of C. elegans. From a pilot insertiona...

We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluoresc...

Projecting from most cell surfaces, cilia serve as important hubs for sensory and signaling processes have been linked to a variety of human disorders, including Bardet-Biedl Syndrome (BBS), Meckel-Gruber (MKS), Nephronophthisis (NPHP), Joubert Syndrome, these diseases are collectively known ciliopathy. DCDC2 is ciliopathy protein that localizes cilia; nevertheless, our understanding the role i...

Abstract Cellulose is a potential source of fermentable sugars and promising cost-effective for biofuel production. This study aimed to obtain cellulolytic fungi from the intestine Asiatic rhinoceros beetle ( Oryctes L.). The were isolated using Potato Dextrose Agar (PDA) media spread plate method. Qualitative quantitative screening tests carried out 1% CMC (w/v) as sole carbon source. Twelve i...

The coconut palm (Cocos nucifera L.) is a common crop in pantropical areas facing various challenges, one of them being the control diseases and pests. Diseases such as bud rot caused by Phytophthora palmivora, lethal yellowing phytoplasmas types 16SrIV-A, 16SrIV-D or 16SrIV-E, among others, pests like weevil, Rhynchophorus vulneratus (Coleoptera: Curculionidae), horned beetle, Oryctes rhinocer...

Caenorhabditis elegans (C. elegans), a member of the phylum Nematoda, carries the evolutionarily conserved genes comparing to mammals. Due to its short lifespan and completely sequenced genome, C. elegans becomes a potentially powerful model for mechanistic studies in human diseases. In this mini review, we will outline the current understandings on C. elegans as a model organism for microRNA (...

Stearic acid represents one of the most abundant fatty acids in Western diet and profoundly regulates health diseases animals human beings. We previously showed that stearic supplementation promoted development terrestrial model nematode Caenorhabditis elegans chemically defined CeMM food environment. However, whether other nematodes remains unknown. Here, we found dietary with could promote ma...

during a survey, five known species of plant-parasitic nematodes of the families tylenchidae and dolichodoridae (tylenchomorpha: tylenchoidea) were collected and identified from several locations in golestan, guilan, kurdistan, and fars provinces. paratrophurus kenanae, tylenchus elegans and t. ritae, as new records for iranian nematode fauna, with the two previously reported species filenchus ...