To gain further information on the mechanisms by which the fluorinated pyrimidines and their nucleosides inhibit various processes of nucleic acid biosynthesis, studies similar to those re ported in the preceding paper (6) were carried out in vitro in glycolyzing suspensions of the Ehrlich ascites cells system used by LePage for studies of purine biosynthesis (20). In this work the following la...

The experiments performed in this report were designed to investigate the mechanisms involved in the metabolic alterations associated with orotic acid-induced hepatic steatosis and the effect of fenofibrate, a stimulant of peroxisome proliferators-activated receptor alpha (PPARalpha), on these alterations. Male Wistar rats were divided into three experimental groups: 1) fed a balanced diet (C);...

A possible relationship of RNA metabolism to higher brain functions has been inferred from theoretical considerations 9, from experiments in which RNA-blocking antimetabolites affect behavior 2, and from reports that training affects either the rate of labeling of brain RNA by radioactive precursors 24, or changes the patterns of labeling la. An indication of such a qualitative change from inje...

The composition of fourteen infant formulae and six follow-up milks with regard to their free amino acids (including taurine), free nucleotides, orotic acid, and free and total l-carnitine content was studied. The levels found were compared with the limits established in European legislation and with the composition of human and cows' milk samples. HPLC methodologies, optimized and validated fo...

A line of Gardner lymphosarcoma resistant to 5-fluorouracil (FU)' was derived by treating the drug-sensitive line with FU for @1 selection passages. There was a signifi cant decrease in orotic acid decarboxylating enzyme activity in the resistant tumor line as compared with that in the drug-sensitive line. A partially purified preparation of OMP-pyrophosphorylase from drug-sensitive tumor cells...

WADA, Y., NISHIMURA, Y., TANABU, M., YOSHIMURA, Y., IINUMA, K., YosHIDA, T. and ARAKAWA, Ts. Hypouricemic, Mentally Retarded Infant with a Defect of 5-Phosphoribosyl-I-pyrophosphate Synthetase of Erythrocytes. Tohoku J. exp. Med., 1974, 113 (2), 149-157 An infant with hypouricemia due to a defect of 5-phosphoribosyl-l-pyrophosphate synthetase (PRPP synthetase) (E.C.2.7.6.1.) was described, who ...