Organogenesis and somatic embryogenesis have been widely applied as the two main regeneration pathways in plant tissue cultures. However, recalcitrance is still restriction clonal propagation of many woody species, especially conifers. They undergo a “phase change” that leads to significant loss vegetative capacity, reducing aptitude tissues organs be regenerated vitro beyond this point. In lin...

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HIGHLIGHTS Agar is the most used gelling agent for plant culture medium, but it expensive. Gelling of medium with κ-carrageenan improves in vitro growth and direct indirect organogenesis Comanthera mucugensis. The use represents a 23.2% reduction cost producing compared agar. New studies are needed to improve rooting C.