background: addition of amino acids to the culture medium is beneficial for embryonic development in many species. objective: the objective of this study was to investigate the effects of amino acids on the in vitro maturation and embryonic development of the bovine oocyte. materials and methods: bovine ovaries were collected from a local abattoir and brought into laboratory. cumulus-oocyte com...

The toxicity of QD has been extensively studied over the past decade. However, the potential toxicity of QDs impedes its use for clinical research. In this work, we established a preantral follicle in vitro culture system to investigate the effects of QD-Transferrin (QDs-Tf) bioconjugates on follicle development and oocyte maturation. The preantral follicles were cultured and exposed to CdTe/Zn...

OBJECTIVE To assess the impact of prior unilateral or bilateral endometrioma cystectomy on controlled ovarian hyperstimulation (COH) and intracytoplasmic sperm injection (ICSI) outcome. DESIGN Retrospective case-control study. SETTING Department of Obstetrics and Gynecology, School of Medicine, Hacettepe University, Ankara, Turkey. PATIENT(S) Fifty-seven consecutive infertile patients wer...

The aim of the present study was to evaluate the efficiency of different doses of epidermal growth factor (EGF) on maturation and fertilisation of bovine oocytes in vitro. A total of 1209 high quality oocytes (follicles 2-8 mm in diameter, with unexpanded cumulus cells) were obtained by aspiration from ovaries collected at a slaughterhouse. The oocytes were then assigned into 6 groups: 1, 10, 1...

This study was performed to investigate the effect of of transdermal testosterone gel (TTG) on controlled ovarian stimulation (COS) and IVF outcomes and ovarian morphology according to pretreatment duration in poor responders. A total of 120 women were recruited for this pilot study. They were randomized into control, 2 weeks, 3 weeks or 4 weeks TTG treatment groups. For three TTG treatment gro...

Newt oocytes were dissected under liquid paraffin and known volumes of clean nucleoplasm and cytoplasm were obtained from oocytes in different stages of oogenesis. The samples were digested in redistilled nitric acid, diluted with deionized water, and their Na and K contents were measured by flame spectrophotometry. The results were expressed as micro-equivalents of Na and K per millilitre of n...

We have previously demonstrated that activin is likely an ovarian mediator of pituitary gonadotropin(s) and local epidermal growth factor in their stimulating oocyte maturation and maturational competence in the zebrafish. However, the downstream events controlled by activin remain unknown. One possible mechanism is that activin may directly work on the oocytes to promote the development of ooc...

This study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (IVM) and in vitro fertilization (IVF) rate of buffalo oocytes. Cumulus oocytes complexes (COCs) were aspirated from follicles of 2-8 mm diameter. In experiment I, COCs were matured in IVM medium supplemented with 0 (control), 250, 500, and 1000 μM melatonin for 22-24 hours in CO2 in...

Background: In vitro maturation (IVM) of oocytes reduces the costs and averts the side-effects of gonadotropin stimulation for in vitro fertilization (IVF). Reliable IVM is an intellectual, scientific and clinical challenge with a number of potential applications. Objective: The effect of hCG was evaluated on the timing and regulation of in vitro ovulation for the Syrian mice oocytes in the pre...

In the current study, hypothesis of effects luteinizing hormone (LH) and follicular fluid (FF) derived from follicles varying size on in vitro embryo production Shami goat breed were tested. The caprine (cFF) was obtained healthy female’s ovaries by aspiration method classified into two main classes (follicles with a diameter ≤ 2mm ≥3mm). resulting cFF added to culture medium TCM-199 through si...