This study combines microfluidics with optical microablation in a microscopy system that allows for high-throughput manipulation of oocytes, automated media exchange, and long-term oocyte observation. The microfluidic component of the system transports oocytes from an inlet port into multiple flow channels. Within each channel, oocytes are confined against a microfluidic barrier using a steady ...

in the present study, effect of five isolates of trichoderma harzianum on growth parameters of tomato was carried out as cr design with six replications under greenhouse conditions. the concentrations 0.5%, 1% and 1.5% inoculums per pot were used. plant length, shoot and root fresh/dry weight, number of leaves and stem diameter as growth parameters were measured. the results showed that isolate...

it has often been stated or implied that grazing is an important factor in the land degradation process, a suggestion supported by various research projects, and this study investigated the effect of livestock grazing on stem height, crowncover, production, basal diameter, stem biomass, litter, leaf-stem index (leaf to stem weight) , root diameter and weight and seed production per individual o...

Bovine ovaries offer a large pool of oocytes that could be used for in vitro production of embryos of genetically valuable animals. The effects of in vitro growth (IVG) culture duration (10, 12, and 14 days) on the viability and growth of bovine oocytes derived from early antral follicles (0.5-1 mm diameter) in this study. In addition, the effect of pre-IVM culture with phosphodiesterase inhibi...

The dictyate nucleus of the growing mouse oocyte exhibits, besides the nucleolus, 3 ultrastructurally distinct types of smaller formed entities referred to as extranucleolar bodies. The extranucleolar bodies of the first type (fibrillogranular bodies) consist of intermingled masses made up of closely arranged convoluted fibrils, 6-10 nm in width, interspersed with electrondense granules approxi...

background: there has been an increase in number of obese infertile females booked for advanced infertility treatment procedures like in vitro fertilization (ivf) and intra cytoplasmic sperm injection (icsi). the knowledge of impact of body mass index (bmi) on reproductive outcome can help to counsel these patients. objective: to compare reproductive outcome in females of different bmi after ic...

several plant growth regulators are used to improve the quality of cut flower in bulb plants, but their effectiveness depends on genotype and plant growth conditions such as nutrition status. a pot experiment was conducted under farm condition to study benzyl adenine (ba) and potassium nitrate interaction on floret diameter, stem diameter, flower height, flower number per bulb, fresh and dry we...

Background Fertility preservation for female cancer patients prior to cancer therapy has emerged as an essential component of comprehensive patient care. In the US, approximately 6% of women diagnosed with invasive cancer between 2007 and 2011 that were under 45 years old. The incidence of cancers in reproductive age women signifies a need for fertility preservation options and this need is inc...