نتایج جستجو برای: one primer pair as1iiamyc5r

تعداد نتایج: 2110471  

Journal: :Journal of agricultural and food chemistry 2003
Yu-Ling Sun Chich-Sheng Lin

A method of fluorescent Polymerase Chain Reaction-restriction fragment length polymorphism (PCR-RFLP) was applied as an analytical and quantitative tool for meat identification. Following alignments of the nucleotide sequences, an oligonucleotide primer pair was designed to amplify the partial sequences within the 12S ribosomal RNA (12S rRNA) gene of mitochondrial DNA from porcine, caprine, and...

Journal: :Journal of clinical microbiology 2001
V N Loparev R F Massung J J Esposito H Meyer

A restriction fragment length polymorphism (RFLP) assay was developed to identify and differentiate Old World, African-Eurasian orthopoxviruses (OPV): variola, vaccinia, cowpox, monkeypox, camelpox, ectromelia, and taterapox viruses. The test uses amplicons produced from virus genome DNA by PCR with a consensus primer pair designed from sequences determined for the cytokine response modifier B ...

2010
Athanasia Spandidos Xiaowei Wang Huajun Wang Brian Seed

PrimerBank (http://pga.mgh.harvard.edu/primerbank/) is a public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR). A total of 306,800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as T(m), location on the transcript and amplic...

Journal: :The Malaysian journal of pathology 2007
I Latifah K y Teoh K L Wan Y Normaznah M Rahmah

Giardia duodenalis causes diarrhoea and malabsorption. The objectives of the study were to detect local isolates of G. doudenalis by polymerase chain reaction (PCR) and to determine their restriction fragment length polymorphisms (RFLP). G. doudenalis isolated from stools of patients from Hospital Orang Asli Gombak were cultured axenically using TYI-S-33 medium with 10% foetal calf serum. The c...

Journal: :Molecular and cellular probes 2000
P Feng S R Monday

A multiplex PCR assay was developed which allowed the simultaneous detection of five trait genes or virulence markers in enterohemorrhagic Escherichia coli (EHEC) serotypes. A primer pair, designed to detect a single base-pair mutation in the uidA gene, is specific only for the prototypic EHEC of O157:H7 serotype and its toxigenic, non-motile variants. In a similar way, primers to the eaeA gene...

2016
María I. Vaquero-Sedas Miguel A. Vega-Palas

This Article contains a typographical error in the Methods section under the subheading 'MMQPCR amplifica-tion' , where " 4 × 1.5 μ L each primer 10 mM (TelA, TelB and the pair of primers used to amplify CYP5) " should read: " 4 × 1.5 μ L each primer 10 μ M (TelA, TelB and the pair of primers used to amplify CYP5) ". This work is licensed under a Creative Commons Attribution-NonCommercial-NoDer...

Journal: :Applied and environmental microbiology 1998
A Tilsala-Timisjärvi T Alatossava

In the present work, strain-specific PCR primers for Lactobacillus rhamnosus Lc 1/3 are described. The randomly amplified polymorphic DNA (RAPD) technique was used to produce potential strain-specific markers. They were screened for specificity by hybridization with DNA from 11 L. rhamnosus strains. A 613-bp RAPD marker found to be strain-specific was sequenced, and a primer pair specific to L....

2010
ALI RAZA AWAN IKRAM UL HAQ MASROOR ELLAHI BABAR IDREES AHMAD NASIR

A Reverse Transcription-Polymerase Chain Reaction (RT-PCR) technique was applied for the detection of Potato leaf roll polerovirus (PLRV) in dormant potato tubers. A primer pair was designed from the coat protein-encoding fragment of the PLRV genome that amplified a 336-bp product. The amplified product was detected in nucleic acid preparations from leaves and tubers of 5 cultivars and from pur...

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