Transmembrane signaling between intracellular compartments is often controlled by regulated proteolysis. Escherichia coli respond to misfolded or unfolded outer-membrane porins (OMPs) in the periplasm by inducing sigma(E)-dependent transcription of stress genes in the cytoplasm. This process requires a proteolytic cascade initiated by the DegS protease, which destroys a transmembrane protein (R...

The Backward Limb Spectrometer (BLS) onboard the Tiangong-2 (TG-2) space laboratory, first spaceborne limb sounding instrument of China, was successfully launched on 15 September 2016, and its measurements scattered photons sunlight along line-of-sight (LOS) in 290–1000 nm range could be used to derive vertical distribution stratospheric ozone with high resolution. Ozone profiles a resolution o...

Although many periplasmic folding factors have been identified, the mechanisms by which they interact with unfolded outer membrane proteins (OMPs) to promote correct folding and membrane insertion remain poorly understood. Here, we have investigated the effect of two chaperones, Skp and SurA, on the folding kinetics of the OMP, PagP. Folding kinetics of PagP into both zwitterionic diC12:0PC (1,...

Onboard both the Suomi National Polar-orbiting Partnership and Joint Polar Satellite System (JPSS) series of satellites, Ozone Mapping Profiler Suite Nadir Mapper (OMPS-NM) is a new generation total ozone column sensor used to generate products. This study presents method for efficiently assessing OMPS-NM geolocation accuracy using spatially collocated radiance measurements from Visible Infrare...

The Joint Polar Satellite System (JPSS) mission has provided over ten years of high-quality data products for environment forecasting and monitoring through the current Suomi National Polar-orbiting Partnership (S-NPP) NOAA-20 satellites. Particularly, sensor record (SDR) derived environmental (EDR) from Visible Infrared Imaging Radiometer Suite (VIIRS), Cross-track Sounder (CrIS), Advanced Tec...

Bacterial surface proteins are involved in direct contact with host cells and in uptake of nutrients from the environment (1). For this reason, cellular localization can provide insights into the functional role of bacterial proteins. Surface localization of bacterial proteins is a key step towards identification of virulence factors involved in mechanisms of pathogenicity. Methods for fraction...

Gram-negative bacteria depend on their cell membranes for survival and environmental adaptation. They contain two membranes, one of which is the outer membrane (OM), home to several different proteins (Omps). One class important Omps porins, mediate inflow nutrients antimicrobial drugs. The microorganism’s sensitivity antibiotics, are predominantly targeted at internal sites, greatly influenced...