We have studied a naturally occurring small-molecule antimitotic called diazonamide A. Diazonamide A is highly effective at blocking spindle assembly in mammalian cell culture and does so through a unique mechanism. A biotinylated form of diazonamide A affinity purifies ornithine delta-amino transferase (OAT), a mitochondrial enzyme, from HeLa cell and Xenopus egg extracts. In the latter system...

Phytochrome was isolated from etiolated rice seedlings (Oryza sativa L.) by slight modification of the procedure for oat phytochrome. Spectral data of rice phytochrome are comparable with those of oat and rye phytochrome. Controlled proteolysis with endoproteinases Lys-C and Glu-C yielded defined fragments some of which were different for Pr and Pfr. The fragments were identified by comparison ...

BACKGROUND Numerous surgical options exist to treat chondral lesions in the knee, including microfracture (MFx), osteochondral autograft transplantation (OAT), first-generation autologous chondrocyte implantation (ACI-1), and next-generation ACI (ACI-2). PURPOSE To compare the cost-effectiveness of MFx, OAT, and ACI-1. The secondary purpose of this study was to compare the functional outcomes...

McCartney, D. H. and Vaage, A. S. 1994. Comparative yield and feeding value of barley, oat and triticale silage. Can. J. Anim. Sci. 74: 91-96. The yield and feeding value of barley (Hordeum vulgare 'Johnson'), oat (Avena sativa 'Calibre') and triticale (Triticosecale 'Carmen') silages were compared in a field study, a heifer growth trial (1 19 d in length, with four pens of eight Charolais cros...

We examined T-cells, B-cells, and natural killer cells of four normal individuals for surface-bound fibronectin (FN) using fluorescence-activated cell sorter analysis and double fluorescence labeling with monoclonal antibodies. While Leu 12 (CD 19)-positive B-cells stained also uniformly with anti-FN antibody, neither OKT 3 (CD 3)-positive T-cells nor Leu 11 (CD 16)-positive natural killer cell...

Oat (Avena sativa L.) traditionally has been a major crop for feed and forage in Turkey. Theobjective of this research was to study hay yield and quality of oat genotypes harvested at thelate milk stage. One hundred oat varieties of worldwide origin were compared in fieldexperiments in Samsun (northern Turkey) over two growing seasons (2007-2008 and 2008-2009).Significant differences between th...

The goal of this paper is to compare and contrast various image reconstruction algorithms for optoacoustic tomography (OAT) assuming a finite linear aperture of the kind that arises when using a linear-array transducer. Because such transducers generally have tall, narrow elements, they are essentially insensitive to out of plane acoustic waves, and the usually 3D OAT problem reduces to a 2D pr...

Understanding relationships between sequence, structure, and evolution is important for functional characterization of proteins. Here, we define a novel DOM-fold as a consensus structure of the domains in DmpA (L-aminopeptidase D-Ala-esterase/amidase), OAT (ornithine acetyltransferase), and MocoBD (molybdenum cofactor-binding domain), and discuss possible evolutionary scenarios of its origin. A...

Three oat cultivars and one oat breeding line were evaluated for chemical, hydration and pasting properties. Protein, starch and β-glucan levels ranged 11.13∼14.37, 56.37∼64.86 and 3.44∼4.76%, respectively. The oat cultivars Daeyang and Seonyang contained higher β-glucan levels of 4.76 and 4.35%. The Daeyang variety had a higher water absorption index (WAI) of 2.83∼3.35 (g/g), but a lower water...

Cold acclimation protects plants from temperate regions of the world from the deleterious effects of low and freezing temperatures. This is through a series of transcriptional, regulatory and metabolic changes that enable continued growth and survival. The focus in this thesis is to increase our understanding of the cold acclimation process and there by open the door to development of cold hard...