A continuous-flow clinical analyzer for the routine estimation of urea is described that makes use of an immobilized-enzyme nylon-tube reactor as part of a flow-through system (a Technicon AutoAnalyzer I). Results of blood-urea analyses by use of the immobilized urease are compared with determinations made with the diacetyl monoxime method and the urease solution method. Clinical trials carried...

We describe a procedure for recycling nylon hybridization membranes, enabling their repeated use for radioactive Southern hybridization analysis of different DNA samples. Following hybridization and probe removal, nylon membranes containing covalently linked DNAs were treated with 0.55% sodium hypochlorite. This destroyed the DNA, thereby preventing it from participating in further hybridizatio...

We describe routine methods for determining glucose in plasma with use of aldehyde dehydrogenase or glucose oxidase-aldehyde dehydrogenase immobilized in a nylon tube that is integrated into a continuous-flow system. Although the coupled-enzyme nylon-tube reactors require the presence of a third enzyme, catalase, in solution, the kinetics are not so complicated as to preclude reliable routine d...

The Hader bar system is a popular bar and clip concept because of its 20-degree clip rotation, simplicity, and versatility. Two procedures may be used to incorporate the Hader nylon clip into a denture base. The direct technique consists of attaching the clip to the denture base as a clinical procedure. With the indirect technique, the clip is attached during laboratory processing. An alternati...

Print-capture (PC) Polymerase chain reaction (PCR) was evaluated as a novel detection method of plant viruses. Tomato (Lycopersicon esculentum) plants infected with begomovirus (fam. Geminiviridae, gen. Begomovirus) and viruliferous whiteflies were used to study the efficiency of the method. Print-capturing steps were carried out using non-charged nylon membrane or filter paper as the solid sup...

We report a method for immobilizing glucose dehydrogenase on the inside surface of nylon tubes to produce an immobilized-enzyme nylon-tube reactor. The glucose dehydrogenase reactor is integrated into the flow system of a continuous-flow analyzer to facilitate routine analysis of serum glucose at 50 samples/h. We compared results with those by the reference hexokinase/glucose-6-phosphate dehydr...

the nylon and nylon/lycra yarns were coated with electrically conductive polymers such as polyaniline and polypyrrole, via chemical polymerization process. electrical conductivity of the coated yarns was measured at variousstrain levels using two-point probe technique and their strain sensitivities were studied. the results showed that, electrical conductivity of the coated yarns decreased with...

A plastic bag method was developed to observe air-sensitive samples on microstructure and phase distribution without exposure to air during the holder transfer process into the transmission electron microscope (TEM). As an example, a type of lithium aluminum hydride (Li(3)AlH(6)) was observed in the TEM to demonstrate the effectiveness of this method. Results show that the plastic bag method is...

The process parameters of FDM manufacturing technology are very important for obtaining high quality parts, good mechanical properties. Several optimization techniques used such as Response surface methodology, Taguchi method, FF, ANN etc. much research work has been carried out in ABS, PC, PPSF, Nylon-12, PC-ABS blend for optimizing process parameters to identify improved surface roughness, me...