Final oocyte maturation using GnRH-agonist trigger in a GnRH-antagonist protocol is increasingly common, as ovarian hyperstimulation syndrome is almost completely avoided. However, this approach might lead to reduced pregnancy rates due to severe luteolysis. This proof of concept study evaluated the extend of luteolysis by measuring progesterone levels 48 hours after oocyte retrieval in 51 pati...

This study investigated the effect of different harvesting techniques on the recovery rate and quality of bovine cumulus oocyte complexes (COCs) and their subsequent developmental competence including in vitro maturation, fertilization and blastocyst formation. The COCs were retrieved from slaughter ovaries using aspiration, slicing, or aspiration followed by slicing. The COCs were examined mic...

Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...

Introduction: Genital system is important herd in animal's creation. It is necessary for animal reproduction. Mammal has two sex in genital system such as male and female in separated animals. Animal reproduction destroyed by disorder of genital system. In this case, animals cannot produce an animal similar to them. Finally it cause overthrow a species of animals. Methods: Histomorpholog...

Background: The developmental competency of embryos is affected by several factors, including the developmental pathways and their elements. In mammalian species including goat, fertilized oocyte undergoes several divisions to form a structure called blastocyst. These events depend on the successful control of temporal and spatial expression of genes involved in genome activation. One of the cr...

Background: The objectives of this study were: a) to observe the influence of cumulus investment expansion on the nuclear chromatin configuration of canine oocytes matured in vitro; b) to examine the relationship between cumulus cell (CC) expansion and its morphology after in vitro maturation (IVM); c) to ascertain the influence of in vivo serum progesterone (SP) concentrations of ovary donors ...

objective: retinoids are recognized as important regulators of cell differention and tissue function. previous studies, performed both in vivo and in vitro, indicate that retinoids influence several reproductive events. in this study, we investigated the effect of all-trans retinoic acid (t-ra) on maturation and fertilization rate of immature oocytes (germinal vesicle). materials and methods: g...