نتایج جستجو برای: nucleus pulposus cells

تعداد نتایج: 1463800  

Journal: :Turkish Journal of Biology 2022

Particular somatic cells, namely cumulus cells (CCs) that support the oocyte maturation, fertility, and viability by providing nutrients energy to envelop mammalian oocyte. In this study, discarded human tissues were used reveal value of hyaluronic acid-rich CCs on several cellular events, including differentiation. Conditioned media, recovered from primary culture CCs, introduced nucleus pulpo...

2016
Haobo Jia Jianxiong Ma Jianwei Lv Xinlong Ma Weiguo Xu Yang Yang Aixian Tian Ying Wang Lei Sun Liyan Xu Lin Fu Jie Zhao

To investigate the mitigation effect and mechanism of oestrogen and PTH on disc degeneration in rats after ovariectomy, as well as on Wnt/β-catenin pathway activity, thirty 3-month-old rats were ovariectomized and divided into three groups. Ten additional rats were used as controls. Eight weeks later, the rats were administered oestrogen or PTH for 12 weeks, and then discs were collected for te...

2017
Xiangyu Deng Wei Wu Hang Liang Donghua Huang Doudou Jing Dong Zheng Zengwu Shao

Purpose To explore the effect and possible mechanism of icariin, a prenylated flavonol glycoside derived from the Chinese herb Epimedium sagittatum that was applied to IL-1β pretreated human nucleus pulposus (NP) cells. Methods Human NP cells were isolated from intervertebral discs of patients with scoliosis and lumbar spondylolisthesis. The cells were divided into five groups: A (blank contr...

2012
Richard Kasch Birger Mensel Florian Schmidt Wolf Drescher Ralf Pfuhl Sebastian Ruetten Harry R. Merk Ralph Kayser

PURPOSE To evaluate changes in nucleus pulposus volume as a potential parameter for the effects of disc decompression. METHODS Fifty-two discs (T8 to L1) were extracted from 26 pigs and separated into thoracic (T8 to T11) and thoracolumbar discs (T12 to L1). The discs were imaged using 7.1 Tesla ultrahigh-field magnetic resonance imaging (MRI) with acquisition of axial T2-weighted turbo spin-...

Journal: :Spine 2003
Ronjon Paul Rex C Haydon Hongwei Cheng Akira Ishikawa Nikola Nenadovich Wei Jiang Lan Zhou Benjamin Breyer Tao Feng Purnendu Gupta Tong-Chuan He Frank M Phillips

STUDY DESIGN A new recombinant adenoviral vector expressing Sox9, a chondrocyte-specific transcription factor, was tested in a chondroblastic cell line and primary human intervertebral disc cells in vitro. Direct infection of intervertebral disc cells then was assessed in a rabbit model. OBJECTIVES To deliver a potentially therapeutic viral vector expressing Sox9 to degenerative human and rab...

2013
Claire Cunningham Akshay Srivastava Estelle Collin Sibylle Grad Mauro Alini Abhay Pandit J. Gerard Wall

Degeneration of the intervertebral discs (IVD) is a leading cause of neck and low back pain. Degeneration begins in the central nucleus pulposus region, leading to loss of IVD osmotic properties. Regeneration approaches include administration of matrix-mimicking scaffolds, cells and/or therapeutic factors. Cell-targeting strategies are likely to improve delivery due to the low cell numbers in t...

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