In the past 15 years, nuclear magnetic resonance (NMR) spectroscopy has emerged as one of the principle techniques of structural biology (1, 2). It not only is capable of solving protein structures to atomic resolution but also has the unique ability to accurately measure the dynamic properties of proteins and to probe the process of protein folding (3, 4). However, a major drawback of macromol...

One of the long-standing mysteries in genomic evolution is the observation that much of the genome is composed of repetitive DNA, resulting in inter- and intraspecific variation in nuclear DNA content. Our discovery of a negative correlation between nuclear DNA content and flower size in Silene latifolia has been supported by our subsequent investigation of changes in DNA content as a correlate...

The relationship between measured transverse energy, total charge recovered in the detector, and size of the emitting system is investigated. Using only very simple assumptions, we are able to reproduce the observed binomial emission probabilities and their dependences on the transverse energy. Our results show that the observed scaling can arise due to a combination of finite-size effects and ...

Chao, P G; Bulinski, J C; Ateshian, G A; +Hung, C T Columbia University, New York, NY [email protected] INTRODUCTION Cartilage compression changes cell shape, volume and hydrostatic and osmotic pressure of chondrocytes in situ [1,2]. For example, changes in the osmotic environment of cells have been shown to modulate chondrocyte biosynthesis, however, the mechanosensing mechanisms are not well...

DNA reassociation was used to estimate GC content, size, and complexity of the nuclear genomes of Colletotrichum from maize and sorghum. Melting-temperature analysis indicated that the GC content of the maize pathotype DNA was 51% and that the GC content of the sorghum pathotype was 52%. DNA reassociation kinetics employing S1 nuclease digestion and an appropriately modified second-order equati...

NUCLEAR DNA CONTENT AND GENOME SIZE OF TROUT AND HUMAN In their recent paper, Thomas et al. (1) discussed design, resolution, sensitivity, and reproducibility of the National Aeronautics and Space Administration/American Cancer Society flow cytometer. The results of their study demonstrated high stability and sensitivity of the instrument, which is suitable for detection of near-diploid tumor c...

About 1% of the total RNA of cell lines producing murine leukemia virus is virus-specific RNA. About one-third of the virus-specific RNA is located within the nucleus. The size distribution of virus-specific RNA was determined before and after denaturation. Before denaturation, virus-specific RNA sequences sedimented as a heterogeneous population of RNA molecules, some of which sedimented very ...

By using a relativistic framework and accurate nuclear spectral functions we evaluate the ratio F 3A /AF 3N of deep inelastic neutrino scattering. Parametrizations of this ratio for different values of Q 2 are provided. These results should be useful for taking into account the nuclear effects in analyses of experimental data in neutrino reactions in nuclear targets, and test QCD predictions fo...

Background and Objectives: RCC is one of the most common genitourinary cancers. Accurate prediction of prognosis would be valuable for adjuvant trial design, counseling and effectively scheduling follow up visits. P53 is a tumor suppressor gene that expresses a protein that involved in both cell-cycle ar...