Deinococcus radiodurans R1 exposed to a lethal dose of cadmium shows differential expression of a large number of genes, including frnE (drfrnE) and some of those involved in DNA repair and oxidative stress tolerance. The drfrnE::nptII mutant of D. radiodurans showed growth similar to that of the wild type, but its tolerance to 10 mM cadmium and 10 mM diamide decreased by ~15- and ~3-fold, resp...

Cassava is an important subsistence crop grown only in the tropics, and represents a major source of calories for many people in developing countries. Improvements in the areas of resistance to insects and viral diseases, enhanced nutritional qualities, reduced cyanogenic content and modified starch characteristics are urgently needed. Traditional breeding is hampered by the nature of the crop,...

We characterized new transgenic varieties of poplar with multiple insect-resistant and salt stress tolerant genes. Two insect-resistant Bacillus thuringiensis (Bt) genes, Cry1Ac and Cry3A, and a salt-tolerant gene, Betaine aldehyde dehydrogenase (BADH) were inserted into a vector, p209-Cry1Ac-Cry3A-BADH. The clone of Populus x euramericana was transformed by the vector using the Agrobacterium-m...

This experiment was conducted during the period from August 2004 to April 2005 in the Biotechnology & Genetic Engineering Laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh. In this experiment, an efficient and reproducible protocol for the production of transgenic rice plants was developed by inoculating mature embryo of three indica ric...

Cotton fiber is multigenic trait controlled by number of genes. Previous studies suggest that one of these genes may be responsible for switching cotton fiber growth on and off to influence the fiber quality produced from a cotton seed. In the present study, the Gossypium hirsutum GhEXPA8 fiber expansin gene was introduced into local cotton variety NIAB 846 by using an Agrobacterium-mediated ge...

In an attempt to improve the salt tolerance of rice, we introduced katE, a catalase gene of Escherichia coli, into the indica rice cultivar Kasalath. Transformation was carried out using Agrobacterium tumefaciens strain EHA101 harboring a binary vector pIES6/Hm/katE which contains genes for catalase katE, hygromycine resistance gene HPT and kanamycin resistance gene NPTII in the T-DNA region. W...

Because of Burkholderia pseudomallei's classification as a select agent in the United States, genetic manipulation of this bacterium is strictly regulated. Only a few antibiotic selection markers, including gentamicin, kanamycin, and zeocin, are currently approved for use with this bacterium, but wild-type strains are highly resistant to these antibiotics. To facilitate routine genetic manipula...

Here we present the generation and function of two sets of bacterial plasmids that harbor fluorescent genes encoding either blue, cyan, yellow or red fluorescent proteins. In the first set, protein expression is controlled by the strong and constitutive nptII promoter whereas in the second set, the strong tac promoter was chosen that underlies LacI(q) regulation. Furthermore, the plasmids are m...

A method for transformation of B.napus is presented in this study. The protocol is based on infection of cotyledonary petioles of 5-day-old and hypocotyl segments of 14-day-old seedlings with an Agrobacterium tumefaciens strain LBA 4404 carrying a disarmed binary vector pBI121. The T-DNA region of this binary vector contains the neomycin phosphotransferase II (NPTII) and glucuronidase (GUS) gen...

Chloroplast transformation in wheat was achieved by bombardment of scutella from immature embryos and immature inflorescences, respectively. A wheat chloroplast site-specific expression vector, pBAGNRK, was constructed by placing an expression cassette containing neomycin phosphotransferase II (nptII) and green fluorescent protein (gfp) as selection and reporter genes, respectively, in the inte...