The cap1 genes are required for the synthesis of type 1 capsular polysaccharide (CP1) in Staphylococcus aureus. We previously showed that the cap1 locus was associated with a discrete genetic element in S. aureus M. In this report, we defined the boundaries of the cap1 element by comparing its restriction pattern to that of a corresponding region from the CP1-negative strain Becker. The element...

One new species of the genus Platambus Thomson, 1859 from Ganzi Tibetan Autonomous Prefecture, Sichuan Province (P. brancuccii sp. n.) and one from Chengdu, Sichuan Province (P. korgei sp. n.) are described. They belong to the Platambus semenowi-group sensu Nilsson (2000). Important species characters (median lobes and colour patterns) of the two new species are figured, and notes on their dist...

BACKGROUND Melioidosis is less common in children than adults. The clinical spectrum of disease varies greatly between the 2 groups. Treatment guidelines are currently based on adult studies, and revision of existing guidelines is necessary to instruct specific pediatric management. METHODS Culture-confirmed cases of melioidosis in the Northern Territory between 1989 and 2013 were identified ...

TO THE EDITOR: Patients with BRAF-mutated metastatic melanoma benefit greatly from the novel BRAF inhibitor dabrafenib and the checkpoint inhibitor ipilimumab, recently listed under the Australian Pharmaceutical Benefits Scheme (PBS). Dabrafenib results in rapid responses; but the cancer later adapts, and patients relapse rapidly. Conversely, ipilimumab slowly reactivates anticancer immunity, m...

Clustering in perinatal data can violate assumptions of independence, an important consideration for data analysis. Few published studies report on the extent of repeat births in routinely collected Australian perinatal data and the implications thereof for analysis and interpretation. This paper reports on a case study that examined the extent and implications of clustering in the Northern Ter...

This article has been retracted at the request of Microbiology because identical bands for the 16S rRNA probe controls in the Northern blots were reported to correspond to experiments using different strains and experimental conditions in articles published in this journal and in Journal of Bacteriology over a period of 5 years.

We screened for new structural non-coding RNAs (ncRNAs) in the genome sequence of the yeast Saccharomyces cerevisiae using computational comparative analysis of genome sequences from five related species of Saccharomyces. The screen identified 92 candidate ncRNA genes. Thirteen showed discrete transcripts when assayed by northern blot. Of these, eight appear to be novel ncRNAs ranging in size f...

Transcriptome analysis has been a key area of biological inquiry for decades. Over the years, research in the field has progressed from candidate gene-based detection of RNAs using Northern blotting to high-throughput expression profiling driven by the advent of microarrays. Next-generation sequencing technologies have revolutionized transcriptomics by providing opportunities for multidimension...

Mechanisms underlying the circadian rhythmicity in intestinal sugar absorption remain unclear. To test whether this rhythmicity is caused by changes in Na(+)-glucose cotransporter 1 (SGLT-1) function, we measured phloridzin-inhibitable sugar fluxes as an index of SGLT-1 activity. Jejunum obtained from rats killed at 6-h intervals during a 12-h light-dark cycle (CT0 is circadian time 0 h, time o...

1050 BioTechniques Vol. 26, No. 6 (1999) DNA in solution were used instead of 10.75 μL of ddH2O and 8.5 μL of DNA in solution. The re-amplified eluted band was electrophoresed, gel-purified and cloned into a pGEM-T vector (Promega, Madison, WI, USA). We then identified clones containing recombinant plasmids, isolated plasmid DNA and used the insert of an individual clone as a hybridization pro...