There are several differentiation methods for mesenchymal stem cells (MSCs) into hepatocyte-like cell. Investigators reported various hepatic differentiation protocols such as modifying culturing conditions or using various growth factors/cytokines. In this literature review, we compared different MSCs extraction and isolation protocols from Wharton’s jelly (WJ) and explored various MSCs differ...

BACKGROUND When studying and designing an artificial bone in vitro with similar features and functionality of natural bone by tissue engineering technology, the culturing environment, especially the mechanical environment is supposed to be an important factor, because a suitable mechanical environment in vitro may improve the adaptability of the planted-in tissue engineering bone in the body. U...

PURPOSE To study the kinetics of growth and the phenotype of cells cultured from human limbal explants in a cholera toxin-free medium with no feeder cell layer. METHODS Human organ-cultured corneas were used to prepare limbal explants (full-thickness and superficial limbal explants) and corneal stromal explants. Cell growth kinetics and phenotypes were assessed by cultivating explants in chol...

nineteen lymph node biopsies, 3 curetting, 15 blood samples obtained from patients with lymphadenitis and high titers of toxoplasma antibodies, and 27 tonsillar tissues removed from patients because of chronic tonsillitis were inoculated intraperitoneally into mice for isolation of toxoplasma gondii. toxoplasms were isolated from 5 lymph node biopsies, one curettage material, 2 tonsillar tissue...

Herpesvirus recovery from normal and experimentally infected simian tissues prepared into 10 to 20% homogenates was compared with that obtained by explanting these same tissues. The explant procedure was by far the more sensitive of the two methods. As a result, 8 herpesvirus isolations were obtained by conventional virus isolation procedures in contrast to 74 isolates when explants were made f...

This study describes the in vitro propagation and ex acclimatization of Philodendron erubescens pink princess, one most popular ornamental variegated foliage plants. For shoot proliferation, protocorm-like bodies princess were cultured on solid Murashige Skoog (MS) media supplemented with 6-benzylaminopurine (BAP) 1-naphthaleneacetic acid (NAA) at different concentrations. The results revealed ...