This report investigates the comparative in vitro controlled release and transfection efficiencies of pDNA-lipofectamine complex (lipoplex) and pDNA-poly(ethylene imine) complex (polyplex), from a biodegradable polycaprolactone (PCL) film. The effect of molecular weight of gelatin used as a porogen on in vitro release and transfection efficiency was also studied. A sustained release profile was...

The aim of this study was to use CO2 at sub-critical pressures as a tool to sinter 3D, macroporous, microsphere-based scaffolds for bone and cartilage tissue engineering. Porous scaffolds composed of ~200 μm microspheres of either poly(lactic-co-glycolic acid) (PLGA) or polycaprolactone (PCL) were prepared using dense phase CO2 sintering, which were seeded with rat bone marrow mesenchymal strom...

Polycaprolactone (PCL) polymers which illustrate both biocompatibility and resorbability for replacement or bulking of damaged or diseased tissue are important in tissue engineering. Cytocompatibilty of these polymers was assessed on two-dimensional PCL disks and threedimensional PCL solid and PCL hollow microspheres using human uterine mixed leiomyosarcoma (SKUT-1) and hamster ductus deferens ...

The aim of this study was to utilize self-assembled polycaprolactone (PCL)-grafted chondroitin sulfate (CS) as an anticancer drug carrier. We separately introduced double bonds to the hydrophobic PCL and the hydrophilic CS. The modified PCL was reacted with the modified CS through a radical reaction (CSMA-g-PCL). The copolymer without doxorubicin (DOX) was noncytotoxic in CRL-5802 and NCI-H358 ...

To improve well-known titanium implants, pores can be used for increasing bone formation and close bone-implant interface. Selective Laser Melting (SLM) enables the production of any geometry and was used for implant production with 250-µm pore size. The used pore size supports vessel ingrowth, as bone formation is strongly dependent on fast vascularization. Additionally, proangiogenic factors ...

Tissue engineering chamber technique can be used to generate engineered adipose tissue, showing the potential for the reconstruction of soft tissue defects. However, the consequent foreign body reaction induced by the exogenous chamber implantation causes thick capsule formation on the surface of the adipose flap following capsule contracture, which may limit the internal tissue expansion. The ...

For successful bone tissue engineering, a scaffold needs to be osteoconductive, porous, and biodegradable, thus able to support attachment and proliferation of bone cells and guide bone formation. Recently, hydroxyapatites (HA), a major inorganic component of natural bone, and biodegrade polymers have drawn much attention as bone scaffolds. The present study was designed to investigate whether ...

For most tissue engineering applications, surface modification and sterilization of polymers are critical aspects determining the implant success. The first part of this study is thus dedicated to modifying polycaprolactone (PCL) surfaces via plasma treatment using a medium pressure dielectric barrier discharge, while the second part focuses on the sterilization of plasma-modified PCL. Chemical...

Abstract In this study, composite nanofiber films for the wound dressing application were prepared with silk fibroin (SF) and polycaprolactone (PCL) by electrospinning techniques, SF/PCL characterized combined techniques of scanning electron microscopy (SEM), equilibrium water content, Fourier transform infrared spectrometer test, X-ray diffraction (XRD) cell viability test. The results indicat...

Objective: To investigate the effects of growth factors fibroblast-derived extracellular matrix formation in the 3D fiber-deposited polycaprolactone (PCL) scaffold fabricated by 3D printing technique for tissue engineering applications of tunica albuginea. Methods: PCL scaffold was fabricated by 3D bioprinting system. For in vitro cell study, scaffolds were seeded with human fibroblast cell at ...