Pathogenesis of macrovascular complications of diabetes may involve an activation of the transcription factor nuclear factor-kB (NF-kB) by hyperglycemia and advanced glycosylation end products (AGEs). Activation of NF-kB is believed to be dependent on activation of the Rho family of GTPases. Although the precise mechanism of the Rho-mediated action is not completely understood, posttranslationa...

Purpose: NF-kB transcription factor plays a key role in the pathogenesis of multiple myeloma in the context of the bone marrow microenvironment. Both canonical and noncanonical pathways contribute to total NF-kB activity. Recent studies have shown a critical role for the noncanonical pathway: selective inhibitors of the canonical pathway present a limited activity, mutations of the noncanonical...

BACKGROUND AND PURPOSE Reovirus is a ubiquitous RNA virus that exploits aberrant signaling pathways for its replication. The oncolytic potential of reovirus against numerous cancers under pre-clinical/clinical conditions has been documented by us and others. Despite its proven clinical activity, the underlying mechanisms of reovirus oncolysis is still not well elucidated. If reovirus therapy is...

Purpose: NF-kB transcription factor plays a key role in the pathogenesis of multiple myeloma in the context of the bone marrow microenvironment. Both canonical and noncanonical pathways contribute to total NF-kB activity. Recent studies have shown a critical role for the noncanonical pathway: selective inhibitors of the canonical pathway present a limited activity, mutations of the noncanonical...

Interleukin-6 (IL-6) is a multifunctional cytokine expressed by angiotensin II (Ang II)-stimulated vascular smooth muscle cells (VSMCs) that functions as an autocrine growth factor. In this study, we analyze the mechanism for Ang II-inducible IL-6 expression in quiescent rat VSMCs. Stimulation with the Ang II agonist Sar Ang II (100 nmol/L) induced transcriptional expression of IL-6 mRNA transc...

Despite extensive investigations, restenosis, which is characterized primarily by neointima formation, remains an unsolved clinical problem after vascular interventions. A recent study has shown that CD40 signaling through TNF receptor associated factor 6 (TRAF6) plays a key role in neointima formation after carotid artery injury; however, underlying mechanisms are not clearly elucidated. Becau...

Psychological stress signaling affects skin via corticotropin releasing hormone (CRH) release. Through receptor CRH-R1, CRH activates inflammation mediators like NF-kB, IL1-β, IL6 and TNF-α in keratinocytes. Inflammation increases redness leads to extracellular matrix degradation, reduced elasticity / firmness, wrinkling. We show mitigation of these effects with an aqueous extract Cistus cretic...

Nuclear factor-kB (NF-kB) is a ubiquitous transcription factor that governs the expression of genes encoding cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NF-kB is activated by several agents, including cytokines, oxidant free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral prod...

In the complex microenvironment where they evolve, developing cells undergo rapid programmed cell death (PCD) when cytokines that support them become limiting. The transcriptional mechanisms of cytokine-withdrawal apoptosis are poorly understood. In this report, we used early B-lymphocyte tissue culture and transgenic cells to demonstrate that nuclear factor-kB (NF-kB) promotes apoptosis during...

ENDOMETRIAL CARCINOMA CELL LINES AND PRIMARY CULTURE EXPLANTS. Xavier Dolcet, David Llobet, Mario Encinas***, Judit Pallares, Albert Cabero*, Joan Antoni Schoenenberger**, Joan X Comella***, Xavier Matias-Guiu Departments of Pathology and Molecular Genetics, Gynecology*, and Pharmacy**, Hospital Universitari Arnau de Vilanova, Cell signalling and Apoptosis Group***. Laboratori de Recerca de l’H...