Acquisition of resistance to neomycin, gentamicin, fusidic acid, or clindamycin has been observed in three strains of Staphylococcus aureus and data from three patients infected with these strains are presented in detail. Clindamycin resistance followed the expected pattern by appearing in a strain of Staph. aureus with dissociated resistance to erythromycin after treatment with erythromycin an...

To investigate the recombinational machinery of mammalian cells, we have constructed plasmids that can be used as substrates for homologous recombination. These plasmids contain two truncated nontandem, but overlapping, segments of the neomycin resistance gene, separated by the transcription unit for the xanthine guanine phosphoribosyl transferase gene. Recombination between the two nonfunction...

Watanabe, Tsutomu (Keio University School of Medicine, Tokyo, Japan), Chizuko Ogata, and Sachiko Sato. Episome-mediated transfer of drug resistance in Enterobacteriaceae. VIII. Six-drug-resistance R factor. J. Bacteriol. 88:922-928. 1964.-The multiple-drug-resistant Escherichia coli strain isolated by Lebek in 1963 was found to transfer resistance to sulfonamide, streptomycin, chloramphenicol, ...

In an attempt to clone the ORF of the nptII gene of Escherichia coli K12 (ATCC 10798), two degenerate primers were designed based on the nptII sequence of its Tn5 transposon. The nptII ORF was placed under the control of the E. coli hybrid trc promoter, in the pKK388-1 vector, transformed into E. coli DH5α ΔrecA (recombinant, deficient strain). Transferred cells were tested for ampicillin, tetr...

Prion protein knockout (PRNP(-/-)) cattle have been developed and may be used to produce bovine material such as serum, collagen, and gelatin. However, genetically engineered animals (GE animals) must not be imported or made commercially available in Japan, because they are not authorized for food use in Japan. We used real-time polymerase chain reaction (real-time PCR) to develop method of det...

Six strains of enteropathogenic gram-negative bacteria were tested for susceptibility to neomycin or oxytetracycline alone and combined in fixed ratios. The minimal inhibitory concentration for the combination was less than one-half of that expected if the antibiotic activities were simply additive. Neomycin alone was more effective against bacteria multiplying in the presence of abundant oxyge...

THE CONCEPT of intestinal antisepsis is not new. I t was under taken without success in the preceding century. Not unti l 1941,20 with the discovery of succinylsulfathiazole, was a practical intestinal antiseptic available. T h e sulfa drugs are bacteriostatic-not bactericidal--agents. Consequently, they must be administered for a considerable period of t ime and the resident organisms must be ...

Ribosomes are made up of parts that interact so strongly that a mutation in one of them can mask the effect of mutation in another. For example, when a mutation to neomycin resistance, which is a ribosome mutation, is introduced into cells carrying a ribosome mutation to spectinomycin resistance, some of the doubly mutant strains were phenotypically sensitive to spectinomycin, even though the m...

The aim of the study was to establish the in vitro sensitivity of S. agalactiae (126 strains), S. dysgalactiae (82 strains), S. uberis (419 strains), and Enterococcus sp. (136 strains) isolated from mastitis in cows to 13 antibiotics. Bacteriological examinations of quarter milk samples were conducted with the recommendations of the IDF. Sensitivity to antibiotics was tested by disk diffusion m...

We have studied the binding of neomycin to a 171mer RNA (psi-RNA) from the packaging region of the LAI strain of human immunodeficiency virus type-1, HIV-1 (LAI). The RNase I footprinting studies reveal that the primary binding site for the drug is in stem-loop 1, which contains the dimer initiation site of HIV-1. Loading this site with neomycin causes a structural change in the RNA, allowing n...