The complete mitochondrial genome of Purpureocillium lavendulum was characterized in this study. This mitogenome is a closed circular molecule 23,567 bp length with GC content 28.46%, including 15 protein-coding genes, 25 transfer RNA 2 ribosomal genes. Phylogenetic analyses based on sequences at the 14 concatenated genes showed that P. closely related to Hirsutella minnesotensis.