introduction: [61cu]diacetyl-bis(n4-methylthiosemicarbazone) ([61cu]atsm) is a well-established hypoxia imaging tracer with simple production and significant specifity. in this work the accumulation of the tracer is studied in wild-type, necrotic and hypoxic fibrosarcoma tumors. methods: [61cu]atsm was prepared using atsm ligand and [61cu]cuoac followed by i.v. administration and imaging studi...

endodontic intervention in necrotic immature permanent teeth is usually a clinical challenge. with appropriate case selection, regenerative treatment can be effective, providing a desirable outcome. however, there is still no consensus on the optimal disinfection protocol or the method to achieve predictable clinical outcome. this article presents two cases of regenerative treatment in necrotic...

Objective. We hypothesized that HMGB1 in complex with bacterial components, such as flagellin, CpG-ODN, and LPS, promotes HIV-1 replication. Furthermore, we studied the levels of antiflagellin antibodies during HIV-1-infection. Methods. Chronically HIV-1-infected U1 cells were stimulated with necrotic extract/recombinant HMGB1 in complex with TLR ligands or alone. HIV-1 replication was estimate...

Gastric mucosal cell death due to various gastric stressors can cause several types of gastric diseases, such as gastric ulcers. In this study, we examined cell death following the short-term treatment of guinea pig gastric mucosal cells in primary culture with various gastric stressors. The short-term treatment of cells with ethanol, hydrogen peroxide or hydrochloric acid caused, in a dose-dep...

Previous studies have shown that complement activation on renal tubular cells is involved in the induction of interstitial fibrosis and cellular injury. Evidence suggests that the tubular cell damage is initiated by the alternative pathway (AP) of complement with properdin having an instrumental role. Properdin is a positive regulator of the AP, which can bind necrotic cells as well as viable p...

AIMS The effect of transpupillary thermotherapy (TTT) on human choroidal melanomas was investigated by means of histopathology. METHODS Before enucleation TTT was performed in 11 eyes with a xenon are photocoagulator with a red filter or a diode laser at 810 nm. The exposure time was 1 minute; the estimated temperature at the top of the tumour was about 65 degrees C. RESULTS Seven of 11 tum...

A unique feature of human alveolar macrophages is their prolonged survival in the face of a stressful environment. We have shown previously that the ERK MAPK is constitutively active in these cells and is important in prolonging cell survival. This study examines the role of the ERK pathway in maintaining mitochondrial energy production. The data demonstrate that ATP levels in alveolar macropha...

Aim: Negative pressure wound therapy (NPWT) has achieved widespread success in the treatment of chronic wounds. However, its effects have been only partially explored, and investigations have generally concentrated on the wound‐dressing interface; a detailed histopathological description of the evolution of wounds under NPWT is still lacking. The present study was performed to investigate the e...

N-Acetylmuramyi-L-alanyl-D-isoglutamine (muramyl dipeptide, MDP) 1 was shown (1) to be the minimal and essential s t ructure requi red for adjuvant activity o f bacterial cell wall peptidoglycans. Fur ther , MDP has been found to possess various biological activities, o ther than adjuvant activity, that are exer ted by bacterial cell wall peptidoglycans. Since MDP is such a potent immunomodula ...