In recent years, there has been much debate about the concept of digital natives, in particular the differences between the digital natives’ knowledge and adoption of digital technologies in informal versus formal educational contexts. This paper investigates the knowledge about educational technologies of a group of undergraduate students studying the course Introduction to eLearning at a univ...

A thermally stable laccase was purified from the culture filtrate of Hexagonia tenuis MTCC-1119. The method involved concentration of the culture filtrate by ammonium sulphate precipitation and an anion-exchange chromatography on diethylaminoethyl (DEAE) cellulose. The sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and native polyacrylamide gel electrophoresis (native-PAG...

Lipases (Triacylglycerol acylhydrolase) are industrially important enzymes that are mainly used in the food and pharmaceutical industry. Lipase enzymes are also used in food materials, detergent, medicine, cosmetics, leather, perfumery and other items. The enzyme was isolated from Myroides odoratimimus and optimized to different physiochemical characteristics, giving its optimum temperature 37°...

AIMS The dosage of fibrinolytic agents such as streptokinase must be controlled carefully to maximize therapeutic activity while avoiding adverse effects. Therefore, the integrity and activity of streptokinase products is likely to be clinically relevant. This study was conducted to compare the in vitro characteristics of different streptokinase preparations. METHODS AND RESULTS Sixteen strep...

Polyhydroxybutyrate (PHB) is a carbon reserve found in some bacteria, and under nutrient limiting conditions accumulates intracellularly in the form of inclusion bodies. These inclusions contain proteins, and the PHI3 within the inclusions exists in an amorphous state. In this study a procedure to recover native PHB inclusions was developed, and the isolated inclusions were characterized using ...

We have purified a 21-kDa protein, designated as P1, from Rehmannia glutinosa to homogeneity by ammonium sulfate precipitation, anion exchange chromatography, hydrophobic interaction chromatography, and preparative native PAGE. The purified P1 had chitin degradation activity. The N-terminal amino acid sequence of P1 indicated that it is very similar to those of thaumatin and other reported thau...

The gene encoding the 45/47 kDa glycoprotein (Rv1860) of Mycobacterium tuberculosis was expressed in Streptomyces lividans under its own promoter and under the thiostrepton-inducible Streptomyces promoter PtipA. The recombinant protein was released into the culture medium and, like the native protein, migrated as a double band at 45 and 47 kDa in sodium dodecyl sulfate (SDS)-polyacrylamide gel ...