This report describes an automated coupled column microbore-high-performance liquid chromatography (HPLC) with fluorescence detection for direct determination of verapamil in small volume of rat plasma. We used HPLC system consisting of three columns such as precolumn, intermediate and analytical column and six-port switching valve and injected small volume of rat plasma to the system without s...

The solvolysis kinetics of a developmental active pharmaceutical ingredient (API) were investigated using a high temperature (HT)-HPLC reactor approach to determine whether it might be possible to use the technique to efficiently screen the relative stabilities of typical APIs (particularly those that are stable at the column temperatures achievable on most HPLC systems and over durations of le...

Highly purified Musca ecdysteroidogenin (ESG) was obtained by extracting housefly heads with an acid-ethanol-urea procedure followed by desalting on a Sephadex G25F column and fractionation on a Sephadex G50F column. Further fractionation was done on a semi-preparative C18 reverse-phase HPLC column with an acetonitrile gradient in 0.1% trifluoroacetic acid in water. Ecdysteroidogenic activity e...

A validated HPLC method was developed to determine doxorubicin concentration in a small volume of rat plasma (60 µl) with convenient fluorescence detection. Sample preparation includes a simple one-step liquid-liquid extraction using minimum amount of organic solvent, with extraction recoveries more than 95%. The analysis was accomplished using PerfectSil C18 column maintained at 35 °C and a mo...

Dissolution of hyperpolarized species in liquids of interest for NMR is often hampered by the presence of bubbles that degrade the field homogeneity. Here a device composed of a bubble pump and a miniaturized NMR cell both fitted inside the narrow bore of an NMR magnet is built by 3D printing. (129)Xe NMR experiments performed with hyperpolarized xenon reveal high and homogeneous dissolution of...

A validated HPLC method was developed to determine doxorubicin concentration in a small volume of rat plasma (60 µl) with convenient fluorescence detection. Sample preparation includes a simple one-step liquid-liquid extraction using minimum amount of organic solvent, with extraction recoveries more than 95%. The analysis was accomplished using PerfectSil C18 column maintained at 35 °C and a mo...

a simple and sensitive high performance liquid chromatographic method with electrochemical detection is described for the quantitative determination of the amoxycillin and clavulanic acid in pharmaceutical dosage forms. separation of sample components occurs on a reversed phase c18 column with a mobile phase consisting of methanol-water-phosphate buffer. for the amperometric detection, the pote...

PAN, C. and HUANG, W., 2010. Numerical modeling of suspended sediment transport in Qiantang River: an estuary affected by tidal bore. Journal of Coastal Research, 26(6), 1123–1132. West Palm Beach (Florida), ISSN 0749-0208. Study of suspended sediment transport in an estuary affected by tidal bore is important for coastal engineering and management because the tidal bore can cause strong sedime...