We investigated the effects of topically administered catecholamines and acetylcholine (ACh) on the cerebrocortical microcirculation and NAD/NADH redox state in chloralose-anesthetized cats. NADH fluorescence of the brain cortex and the volume of small intracortical vessels were measured by fluororeflectometry, and in most of the experiments the pial vessels were photographed simultaneously thr...

NADH increases during ischemia because O(2) shortage limits NADH oxidation at the electron transport chain. Ischemic (IPC) and anesthetic preconditioning (APC) attenuate cardiac reperfusion injury. We examined whether IPC and APC similarly alter NADH, i.e., mitochondrial metabolism. NADH fluorescence was measured at the left ventricular wall of 40 Langendorff-prepared guinea pig hearts. IPC was...

Pyridine nucleotide levels in the corneal epithelium were measured using redox fluorometry, a noninvasive method for monitoring the metabolic status of corneal tissue, and a sensitive bioluminescent assay and an improved extraction procedure that allows the simultaneous extraction and measurement of both NADH and NAD. The same corneas were measured using each of the two methods to enable compar...

Although, starting with Straub’s diaphorase (4), a number of enzymes capable of oxidizing reduced nicotinamide adenine dinucleotide (NADH) have been isolated from respiratory chain preparations during the past 25 years (5-9), it has not been possible to identify even tentatively any of these as a relatively unmodified, soluble form of the primary dehydrogenase of the mitochondrial NADH oxidase ...

The formation ofcis-l,2-dihydroxy-l,2,-dihydronaphthalene from naphthalene by naphthalene oxygenase, purified from Corynebacterium renale ATCC 15075, was demonstrated to involve oxidation of a mol NADH and consumption of one mol oxygen. The enzyme contains one g-atom Fe and one FAD. Catalase inhibited product formation and H2O2 could substitute for NADH in the reaction. Superoxide dismutase inh...

Abstract Two‐photon fluorescence lifetime imaging is a versatile laboratory technique in the field of biophotonics and its importance also growing vivo diagnostics for medical purposes. After years experience dermatology, endoscopic implementations are now posing new technical challenges. To develop, test, compare instrumental solutions this purpose suitable reference samples have been devised ...

The NAD+-specific isocitrate dehydrogenase from pea stems is shown to respond to the mole fraction of NADH when total NAD (NAD+ plus NADH) is kept constant. Fifty per cent inhibition occurs when the mole fraction of NADH is 0.1. In this region the enzyme is extremely sensitive to NADH concentration and it is suggested that this acts in a similar manner to energy charge in other systems. In an a...

The membrane-bound NADH dehydrogenase (E.C. 1.6.99.3) has been solubilized by sodium deoxycholate treatment of membranes and purified 75 fold by column chromatography on Sephadex G-150 and DEAE cellulose in the presence of sodium cholate. The native enzyme has an apparent molecular mass (M r) of 97 000, containing polypeptides of Mr of about 15 000. The pH optimum was at 7.5. The enzyme was spe...

A gradual increase in blood pressure (BP), often attaining hypertensive levels, is common during aging--"age-related hypertension." Therefore, means to prevent or ameliorate this elevated BP safely are important. Although oral B-nicotinamide adenine dinucleotide (NADH), a natural coenzyme, is used principally to treat various neurologic disorders, we wished to investigate whether this agent had...

With respect to cofactor requirements, NADH, and FMNH(2) were equally effective as electron donors for nitrate reductase obtained from leaves of maize, marrow, and spinach, when the cofactors were supplied in optimal concentrations. The concentration of FMNH(2) required to obtain half-maximal activity was from 40- to 100-fold higher than for NADH. For maximal activity with the corn enzyme, 0.8 ...