The synthesis and characterization of cadmium and mercury complexes of selenocyanate of the type [(L)M(SeCN)2] are described, where L is L-Histidine (His) or L-Glycine (Gly) and M is Cd(2+) or Hg(2+). These complexes are obtained by the reaction of 1 equivalent of respective amino acids with metal diselenocyanate precursor in a mixture of solvents (methanol : water = 1 : 1). These synthesized c...

We determined the structure of the rhodopsin mutant N2C/D282C expressed in mammalian cells; the first structure of a recombinantly produced G protein-coupled receptor (GPCR). The mutant was designed to form a disulfide bond between the N terminus and loop E3, which allows handling of opsin in detergent solution and increases thermal stability of rhodopsin by 10 deg.C. It allowed us to crystalli...

Chromosomes in eukaryotes are linear, whereas those of most, but not all, prokaryotes are circular. To explore the effects of possessing a linear genome on prokaryotic cells, we linearized the Escherichia coli genome using the lysogenic lambda-like phage N15. Linear genome E. coli were viable and their genome structure was stable. There were no appreciable differences between cells with linear ...

In 1939 Schoenheimer, Ratner, and Rittenberg (1) employed nn-tyrosine labeled with heavy nitrogen (N15) to study the metabolism of this amino acid and the fate of its amino nitrogen in rats. The present investigation deals with the metabolism in rats of radioactive nn-tyrosine (tyrosine”), labeled in the /3 position with the long lived Cl* isotope of carbon (2).l The labeling of the carbon skel...

Higher education has undergone substantial digital change due to the impacts of COVID-19 pandemic, which altered status quo in education. Universities across globe have initiated and adapted various course delivery modes mitigate this unprecedented situation. Among these responses, online classes continue be most common. Therefore, study examines institutional knowledge management readiness sus...

When Azotobcter vinelandii is grown on molecular nitrogen, it utilizes ammonia without enzymatic adaptation. Furthermore, the distribution of N15 in amino acids derived from cell hydrolysates is the same whether the isotope is supplied to the organism as ammonia or as Nx. These and other findings (1, 2) provide strong evidence that ammonia is the key intermediate in nitrogen fixation by this or...

Cystic echinococcosis (CE) is a high prevalent zoonosis in the central and southern Peruvian Andes. Serum samples (n50)from patients presenting presumptive clinical and radiological diagnosis of CE (group 1), were tested for antibodies against Echinococcus granulosus metacestode using Arc-5 double diffusion assay (DD5), immunoelectrophoresis (IEF), and immunoelectrotransfer blot (EITB) techniqu...