Mononuclear Ru(III) complexes of the type [Ru(LL)Cl2(H2O)] (LL = monobasic tridentate Schiff base anion: (1Z)-N'-(2-{(E)-[1-(2,4-dihydroxyphenyl)ethylidene]amino}ethyl)-N-phenylethanimidamide [DAE], 4-[(1E)-N-{2-[(Z)-(4-hydroxy-3-methoxybenzylidene)amino]ethyl}ethanimidoyl]benzene-1,3-diol [HME], 4-[(1E)-N-{2-[(Z)-(3,4-dimethoxybenzylidene)amino]ethyl}ethanimidoyl]benzene-1,3-diol [MBE], and N-...

We describe a radioimmunoassay of serum cortisol in which 1251-tracerand preconjugated double antibody are used. This simpler method involves direct serum assay, and the majority of reagents are prepared in-house. Within-assay precision (coefficient of variation) of assay for Ortho Diognostic AlA Cortisol I and II was 3.2% (mean = 131 ig/L, n = 10)and2.2% (mean = 405 tg/L, n = 10), respectively...