We demonstrate continuous label-free detection of two cardiac biomarker proteins (creatin kinase and myoglobin) using an array of microfabricated cantilevers functionalized with covalently anchored anti-creatin kinase and anti-myoglobin antibodies. This method allows biomarker proteins to be detected via measurement of surface stress generated by antigen–antibody molecular recognition. Referenc...

Rhabdomyolysis is a pathogenetic cause of acute kidney injury. In such circumstances, not only should therapeutic strategies to replace the failing kidney be implemented, but measures should also be explored to prevent further damage by circulating myoglobin. Volume expansion and forced diuresis have been used, but when a kidney fails, renal replacement therapies are instituted. The techniques ...

Myoglobin was purified from a muscle extract of lace monitor lizard, Varanus varius, by Sephadex G-75, followed by DEAE-cellulose column chromatography. The apomyoglobin was cleaved with cyanogen bromide. The largest fragment was further digested with pepsin, trypsin, and alpha-chymotrypsin. From the amino acid sequence of the cyanogen bromide fragments, together with those of tryptic peptides ...

PATTENGALE,~AUL K., ANDJOHN 0. HOLLOSZY. Augmentation of skeletal muscle myoglobin by a program of treadmill running. Am. J. Physiol. 2 13(3) : 783-785. 1967.-The purpose of these experiments was to obtain information, under controlled conditions, regarding the effect of regularly performed exercise on the concentration of myoglobin in skeletal muscle. Rats were subjected to a vigorous program ...

The kinetics of the reduction of bisulfite by surfactant myoglobin films were examined using chronoamperometry after the overall stoichiometry of the reduction was examined by coulometry. Surfactant films of myoglobin were formed on a graphite (HOPG) electrode. On this electrode, two waves were observed for myoglobin, corresponding to the Fe(III)/Fe(II) and Fe(II)/Fe(I) reductions. Cyclic volta...

A new surface-crossing algorithm suitable for describing bond-breaking and bond-forming processes in molecular dynamics simulations is presented. The method is formulated for two intersecting potential energy manifolds which dissociate to different adiabatic states. During simulations, crossings are detected by monitoring an energy criterion. If fulfilled, the two manifolds are mixed over a fin...

Meat color is an important aspect of a consumer’s purchase decisions regarding meat products. Perceived meatcolor results from the interaction light, detector (i.e., human eye), and numerous factors, both intrinsic extrinsic tothe muscle, that influence chemical state myoglobin. The complex nature these interactions dictates decisionsregarding evaluations be made carefully investigators have ba...

Timely identification of patients with patent infarct-related artery (IRA) may permit rapid triaging to avoid unnecessary repeat thrombolysis or rescue percutaneous coronary intervention (PCI). Various biomarkers have been proposed to assess IRA patency status. Our group found that the ratio of serum myoglobin levels obtainned before and 60-min after initiation of thrombolytic therapy may provi...

Myoglobin is an important intracellular O2 binding hemoprotein in heart and skeletal muscle. Surprisingly, disruption of myoglobin in mice (myo-/-) resulted in no obvious phenotype and normal cardiac function was suggested to be mediated by structural alterations that tend to steepen the oxygen pressure gradient from capillary to mitochondria. Here we report that lack of myoglobin causes a bioc...