Sorbitol (aldose reductase) pathway flux in diabetes perturbs intracellular metabolism by two putative mechanisms: reciprocal osmoregulatory depletion of other organic osmolytes e.g., myo-inositol, and alterations in NADPH/NADP+ and/or NADH/NAD+. The "osmolyte" and "redox" hypotheses predict secondary elevations in CDP-diglyceride, the rate-limiting precursor for phosphatidylinositol synthesis,...

Three discrete loci required for growth on myo-inositol in Rhizobium leguminosarum bv. viciae have been characterized. Two of these are catabolic loci that code for malonate semialdehyde dehydrogenase (iolA) and malonate semialdehyde dehydrogenase (iolD). IolD is part of a possible operon, iolDEB, although the functions of IolE and IolB are unknown. The third locus, int, codes for an ABC transp...

PCOS is a complicated endocrine disorder that mostly affects between 5% and 10% of women who are reproductive age. Obesity, hyperandrogenism, oligo- or anovulation frequent clinical signs.
 Objective: the study was designed to evaluate effect combining Metformin Myoinositol; main insulin-sensitizing drugs on improving symptoms HRQOLQ in women.
 Materials Methods: A prospective, interv...

1. An inositol monophosphatase was purified to homogeneity from bovine brain. 2. The enzyme is a dimer of subunit Mr 29,000. 3. The enzyme hydrolyses both enantiomers of myo-inositol 1-phosphate and both enantiomers of myo-inositol 4-phosphate, but has no activity towards inositol bisphosphates, inositol trisphosphates or inositol 1,3,4,5-tetrakisphosphate. 4. Several non-inositol-containing mo...

A method for the synthesis of chiral 1,2-diamino-1,2-dideoxy-myo-inositol-based bis-pyridyl ligands 3a and 3b from the corresponding myo-inositol precursor is described. These highly functionalized inosamine-bis-pyridyl ligands are expected to provide a useful platform for exploring the relationship between chiral ligand structure and enantioselective olefin oxidation catalyzed by their metal c...

Inositol pyrophosphates (PP-InsPs) are highly phosphorylated molecules that have emerged as central nutrient messengers in eukaryotic organisms. They can bind to structurally diverse target proteins regulate biological functions, such protein–protein interactions. PP-InsPs strongly negatively charged and interact with basic surface patches proteins, making their quantitative biochemical analysi...

A high concentration of free inositol has been found in the semen of a number of species and most of this seems to arise from the accessory glands, not the epididymis (Mann, 1954, 1964; Hartree, 1957). However, appreciable concentrations of myo-inositol occur in the testis (Dawson & Freinkel, 1961), and recent evidence suggests that it is most likely formed there from glucose via glucose-6-phos...

Inositol phospholipids and inositol phosphates mediate well-established functions in signal transduction and in Ca2+ homeostasis in the CNS and non-neural tissues. More recently, there has been renewed interest in other roles that both myo-inositol and its highly phosphorylated forms may play in neural function. We review evidence that myo-inositol serves as a clinically relevant osmolyte in th...

The separation of myo-inositol phosphates by ion pair, reverse-phase high performance liquid chromatography has been investigated. The retention of the inositol phosphates is dependent on both the polarity of the hetaeron utilized and on the pH of the solvent. A method is presented which permits the isocratic separation of multiple forms of inositol phosphates including isomers of myo-inositol ...

Genes involved in the phospholipid synthesis of Saccharomyces cerevisiae, such as PEM1, PEM2, PSS, and INO1, are coordinately repressed by myo-inositol and choline. In order to investigate this regulation, we transformed wild-type yeast with a PEM1 promoter-lacZ fusion and isolated two mutants, named ric1 and ric2 (regulation by myo-inositol and choline), exhibiting decreased PEM1 expression. T...