shigella species are among the common causes of bacterial diarrhoeal diseases. traditional detection methods are time-consuming resulting in delay in treatment and control of shigella infections thus there is a need to develop molecular methods for rapid and simultaneous detection of shigella spp. in this study a rapid multiplex pcr were developed for simultaneous detection of three pathogenic ...

introduction: awareness of the species of shigella has particular importance in the way of dealing with an outbreak, controlling it, and treating patients. the purpose of this study was to use the multiplex pcr method and comparison with the culture method to detect shigella species, as well as analysing the antibiotic resistance pattern in its different species. methods: simultaneously, the fe...

the present study was designed to investigate the prevalence of salmonella species, their molecular characterization and antibiotic resistance in backyard chickens. a total of 1125 samples were collected from backyard chickens in two consequent samplings. in the first part, samples were included of 820 poor recently hatched chicks, hatching residuals, egg shells in the nest floor, cloacal swabs...

background and objectives: pcr is a sensitive assay and could be used as an accurate diagnostic method for detecting various types of microorganisms' genome in low concentration in biological specimens. the demand for sensitive, rapid, safe and easy detection of pcr products has led researchers to a combination of this method with elisa. materials and methods: conserved sequences were selected ...

A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers were designed for detecting O4, H:i, and H:1,2 antigen genes from the antigen-specific genes rfbJ, fliC, and fljB, respectively. These were evaluated in a multiplex PCR assay by using DNAs from S. enterica serovar Typhimurium, 15 other Salmonel...

Ascaris lumbricoides, Trichuris trichiura, and Necator americanus are medically important soil-transmitted helminths (STHs) occurring frequently worldwide including Thailand. Fecal examination using a microscope has been recommended as the gold standard for diagnosis of STH infections, but suffers from low sensitivity. Recently, highly sensitive and specific assays, such as multiplex quantitati...

PURPOSE To detect simultaneously herpes simplex virus type 1 (HSV-1), type 2 (HSV-2), cytomegalovirus (CMV), and varicella-zoster virus (VZV) in ocular specimens suspected of indicating viral infection, and to compare the results of multiplex polymerase chain reaction (PCR) with those of uniplex PCR. METHODS Forty specimens, collected from 33 patients with clinically suspected herpes virus oc...

OBJECTIVE To validate a multiplex polymerase chain reaction (PCR) assay capable of simultaneously screening vitreous biopsy specimens for a panel of common pathogens in posterior uveitis. METHODS A multiplex PCR assay using novel primer sets for cytomegalovirus (CMV), herpes simplex virus (HSV), varicella zoster virus (VZV), and Toxoplasma gondii was developed. The sensitivity of the assay wa...