BACKGROUND AIMS The biodistribution of human MSCs after systemic delivery is incompletely understood. We investigated the changes in cell size and cell surface markers of human MSCs after intravenous (IV) injection in immune competent mice. METHODS Male human MSCs were labeled with fluorescent vital dye PKH67 and tracked after IV administration in C57/BL6 mice. MSCs were tracked in blood and ...

Selective MSCs differentiation protocol into pancreatic beta cells was conducted in the present study using exendin-4 and TGF-beta. Differentiated and undifferentiated MSCs were assessed in experimental type I diabetes in rats. Ninety female white albino rats were included in the study and divided equally (n=15/group) into 6 groups: healthy control, healthy control rats received acellular tissu...

Human mesenchymal stem cells (MSCs) were evaluated for their ability to activate allogeneic T cells in cell mixing experiments. Phenotypic characterization of MSCs by flow cytometry showed expression of MHC Class I alloantigens, but minimal expression of Class II alloantigens and costimulatory molecules, including CD80 (B7-1), CD86 (B7-2), and CD40. T cells purified from peripheral blood mononu...

Human umbilical cord mesenchymal stem cells (hUC-MSCs) are broadly applied in clinical treatment due to convenient accessibility, low immunogenicity, and the absence of any ethical issues involved. However, microenvironment inflammatory tissues may cause oxidative stress induce senescence transplanted hUC-MSCs, which will further reduce proliferation, migration ability, final therapeutic effect...

Mesenchymal stem cells (MSCs)-based therapy shows promise to treat cartilage degeneration, which lacks the regenerative ability in osteoarthritic patients. Differentiation of MSCs into chondrocytes mainly uses a specific differentiation medium. However, biomechanical induction can direct chondrogenesis without chondrogenic medium, particularly with micropattern. Micropattern provides physical a...

We study collections of Message Sequence Charts (MSCs) defined by High-level MSCs (HMSCs) under a new type of concatenation operation called anchored concatenation. We show that there is no decision procedure for determining if the MSC language defined by an HMSC is regular and that it is undecidable if an HMSC admits an implied scenario. Further, the languages defined by locally synchronized H...

OBJECTIVES To evaluate the effect of mesenchymal stem cells (MSCs) and MSCs mixed with Matrixen as a cell carrier on the erectile dysfunction caused by bilateral cavernous nerve crushing injury. MATERIALS AND METHODS White male Sprague-Dawley rats were divided into 4 groups: sham-operated control group (n = 5), bilateral cavernous nerve crushing group (BCNC group, n = 10), BCNC administered w...

objective: mechanism of zoledronic acid on osteoblastic differentiation of mesenchymal stem cells (mscs) has not fully understood. with the knowledge of some drugs mechanism that alter methylation pattern of some genes, the present research sets out to evaluate osterix (osx) promoter methylation pattern during zoledronic acid-induced osteoblastic differentiation of mscs. materials and methods: ...