The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRNA expression. The results showed that the GH mR...

Translational control of gene expression is a mechanism that affects the relationship between mRNA level and protein level. Translation is commonly deregulated in human diseases such as cancer and understanding which mRNAs are targeted for translational deregulation and the mechanisms that mediate such effects is of high importance. Estimates of global translational activity has primarily been ...

quantitative and model-based prediction of security in the architecture design stage facilitates early detection of design faults hence reducing modification costs in subsequent stages of software life cycle. however, an important question arises with respect to the accuracy of input parameters. in practice, security parameters can rarely be estimated accurately due to the lack of sufficient kn...

abstract is not available.

materials and methods in the first stage, no2 pollutant concentrations were measured using the data collected from air pollution monitoring stations. sampling was performed in six stations. the chosen stations have been based on the us environmental protection agency (epa) standards. in the next step, the data were processed using excel software and information was given to the airq model. data...

Gene expression analysis has become an invaluable tool for understanding gene function and regulation. However, global expression analysis requires large RNA quantities or RNA preamplification. We describe an isothermal messenger RNA (mRNA) amplification method, Ribo-SPIA, which generates micrograms of labeled cDNA from 5 ng of total RNA in 1 day for analysis on arrays or by PCR quantification....

Cytochromes P450 3A (CYP3A) and P-glycoprotein (P-gp) are mainly located in enterocytes and hepatocytes. The CYP3A/P-gp system contributes to the first-pass metabolism of many drugs, resulting in a limited bioavailability. During the neonatal period, a shift between CYP3A7, the fetal form, and CYP3A4 occurs in the liver, but data on the expression of the CYP3A/P-gp complex in the intestine are ...

Real-time quantitative PCR (qPCR) is a powerful tool for quantifying specific DNA target sequences. Although determination of relative quantity is widely accepted as a reliable means of measuring differences between samples, there are advantages to being able to determine the absolute copy numbers of a given target. One approach to absolute quantification relies on construction of an accurate s...

Abstract Introduction: Blood cardioplegia (BC) and Custodiol (CC) have been used for a long time in open heart surgery are highly effective solutions. The most controversial issue among these two is whether there any difference between them regarding myocardial damage after ischemia surgery. In this study, autophagy, apoptosis, hypoxia markers were investigated that way we evaluated the differe...