Follicular atresia is a key event in the selection of the ovulatory follicles and occurs during all developmental stages. The aims of the study were to evaluate the follicular population as well as the rates of follicular recruitment and atresia in different strains of mice. Ovaries were obtained from four strains of mice: G1/ Swiss, G2/ F1 Swiss×C57BL/6, G3/ inbred strain C57BL/6, and G4/ F1 C...

Mathematical models of the hypothalamus-pituitary-ovarian axis in women were first developed by Schlosser and Selgrade in 1999, with subsequent models of Harris-Clark et al. (Bull. Math. Biol. 65(1):157-173, 2003) and Pasteur and Selgrade (Understanding the dynamics of biological systems: lessons learned from integrative systems biology, Springer, London, pp. 38-58, 2011). These models produce ...

occurrenceofoxidative stress (os) following in vitro culture is inescapable. therefore, the aim of the present study was to determine the efficacy of coenzyme q 10 (coq 10 ) supplementation medium on developments and total antioxidant capacity (tac) of mouse vitrified pre-antral follicles. fresh and vitrified-warmed isolated preantral follicles from ovaries of 14-16 day s -old mice were cultured...

Abstract Study question Could a hydrogel with similar mechanical properties of reproductive age ovarian cortex support the growth human preantral follicles? Summary answer Yes, our optimized PEGylated fibrin tailored strength provides suitable substrate for follicle survival and growth. What is known already The most challenging part assembling an engineered ovary developing appropriate scaffol...

the aim of the present study was to investigate the effect of follicle stimulating hormone (fsh) on survival, activation and growth of ovine primordial follicles using histological studies. pieces of ovine ovarian cortex were obtained and cultured for 6 days in minimum essential medium supplemented with or without fsh (50 ng/ml). these fragments were then process to be used for histology compar...

This study was conducted to improve the yield of mature oocytes from in vitro-culture of ovarian primary follicles by optimizing follicle retrieval from neonatal mice of different ages. Primary follicles of 75 to 99 μm in diameter were collected daily from 7- to 14-day-old neonatal mice, and subsequently cultured in α-MEM medium. Number of primary follicles isolated, growth of the follicle duri...