introduction: sodium valproate is an anti convulsant drug which has been used to treat epileptic patients for last decades. even a pregnant patient has to use this drug for the treatment of epileptic seizure. the purpose of this investigation is to study the effect of this medicine on the embryo in the mouse. materials and methods: eight pregnant mice were given 200 mg/kg (i.p.) of sodium valpr...

The mechanisms regulating cell division during development of the mouse pre-implantation embryo are poorly understood. We have investigated whether bone morphogenetic protein (BMP) signaling is involved in controlling cell cycle during mouse pre-implantation development. We mapped and quantitated the dynamic activities of BMP signaling through high-resolution immunofluorescence imaging combined...

It is well known that glycoconjugate components of the cell surface and extracellular matrix, play an essential role(s) in many developmental phenomena such as cell differentiation, migration, and cellular interactions. The purpose of this study was to investigate distribution of this macromolecules during differentiation of the notochord and paraxial mesoderm. Formalin fixed paraffin sections ...

Background Recent researches reveal that manual puncturing of the trophectoderm of blastocyst before vitrification, increase the quality of embryo. However, in any of these studies, the importance of blastocoelic fluid and its impact on the formation of three cell lines is not mentioned. Therefore, in the present study, the effect of blastocoelic fluid reduction before vitrification on survival...

Objective Apigenin is a plant-derived compound belong to the flavonoids category and has the protective effects on different cells. The aim was to evaluate the effect of apigenin on the number of viable and apoptotic blastomeres, the zona pellucida (ZP) thickness and the hatching rate of preimplantation mouse embryos exposed to H2O2 and actinomycin D. MaterialsAndMethods 420 two-cell embryos we...

litis study is based on embryotoxic effects of ethanol on embryos and discussing the morphologic and hhtahtgic changes and defects an mouse. tlie female animals were divided in three groups. hie first group untreated as a control group but the second and third group received 10% and 20% solutions of ethanol respectively. animals get use to certain level of ethanol solution and in the 10th day, ...

introduction: the objective of this study was to assess developmental potential of mouse morulae, early and laic blastocysts after vitrification in r2 and r2+vero mediums. material and methods: morulae, early and late blastocysts were obtained from superovulated nmri female mice. embryos in experimental group were suspended in a solution of ethylene glycol, ficoll and sucrose (efs40) for 2 minu...

This article contains data related to the research article entitled "Transcriptional targets of TWIST1 in the cranial mesoderm regulate cell-matrix interactions and mesenchyme maintenance" by Bildsoe et al. (2016) [1]. The data presented here are derived from: (1) a microarray-based comparison of sorted cranial mesoderm (CM) and cranial neural crest (CNC) cells from E9.5 mouse embryos; (2) comp...

The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...

We have used two different experimental approaches to demonstrate topological separation of parental genomes in preimplantation mouse embryos: mouse eggs fertilized with 5-bromodeoxyuridine (BrdU)-labeled sperm followed by detection of BrdU in early diploid embryos, and differential heterochromatin staining in mouse interspecific hybrid embryos. Separation of chromatin according to parental ori...