Background: Declines in semen quality over time are not well understood. It is suspected that infectious disease may affect reproductive function. The aim of this study was to investigate semen quality in men in infertile relationships who were under investigation for couple infertility and attended a clinic for routine semen analysis over a period of 15years. Materials and Methods: We investig...

This work presents a specifically designed spiral type inertial micro-fluidic channel for separating non-motile sperm cell from cellular debris. Demonstration with a mixture of non-motile sperm and red blood cells showed that 82% of sperm could be focused into a separate outlet from the red blood cells.

BACKGROUND The aim of this study was to determine the relationship between human sperm hyperactivation (HA), sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction (AR) of ZP-bound sperm in vitro. METHODS Sperm samples from 129 infertile men were studied. Motile sperm (2 x 10(6)) selected by Pure sperm were incubated with four oocytes in 1 ml human tubal fluid supplemented wit...

There have been numerous studies that suggest that sperm concentrations (sperm counts) are declining in men. However, other studies suggest that sperm counts are not declining or may be increasing in some areas. Although there is disagreement on whether there is a downward temporal trend in sperm counts, the studies provide evidence that sperm counts vary by geographic location. It has been hyp...

BACKGROUND Klinefelter syndrome (KS) is the most common sex chromosomal disorder in males and historically patients have been labeled as sterile. After the introduction of microdissection testicular sperm extraction (micro-TESE), successful sperm retrievals for intracytoplasmic sperm injection (ICSI) have been reported. MATERIALS AND METHODS A retrospective study was undertaken on ten patient...

The incidence of T. vaginalis infection has been increasing around the world over the past decade. This has lead to insight of the importance of this microorganism as a serious problem in reproductive health outcomes including pelvic inflammatory disease, pregnancy complications and an increased risk of HIV acquisition. For natural fertility, not only optimal semen sample that contains a normal...

Extraction with 0 04% (w/v) Triton X-100 removes the flagellar membrane from sea urchin sperm while leaving the motile apparatus apparently intact When reactivated in a suitable medium containing exogenous adenosine triphosphate (ATP), nearly 100% of the sperm are motile and they swim in a manner resembling that of live sperm. Under standard conditions, with 1 mM ATP at 25 degrees C, the reacti...

Prior to ICSI the cumulus-oophorous-complex of the oocyte is commonly remove with impure animal-derived hyaluronidase of low strength. The lower strength of the enzyme, the high concentration of impurities and the necessity of extensive time-dependent mechanical denudation damages of the oocytes, increases the risk of lysing the oocytes and the transfer of animal-derived pathogens. All this can...

Considerable scope exists for the use of AI in camel breeding programmes but the technology is not fully developed. The fertility of both fresh and liquid-stored (24 h; 4°C) dromedary camel semen diluted in either Green Buffer (GB) or INRA-96 (INRA) was assessed. Different insemination doses were investigated: 150 and 300 x 10 motile sperm for fresh sperm, and 150, 300 and 600 x 10 motile sperm...

The aim of this prospective randomized study was to assess the advantages of a new modified intracytoplasmic sperm injection (ICSI) technique called intracytoplasmic morphologically selected sperm injection (IMSI) over the conventional ICSI procedure in the treatment of patients with severe oligoasthenoteratozoospermia. The new procedure consisted of IMSI based on a preliminary motile sperm org...