Outer membrane protein (OMP) CopB, an iron-repressible 81-kDa major OMP of Moraxella (Branhamella) catarrhalis has been a major focus of investigation. To assess CopB as a potential vaccine antigen, we elucidated the degree of antigenic and sequence heterogeneity in this protein among strains of M. catarrhalis. Two monoclonal antibodies, 1F5 and 2.9F, which bind to surface-exposed epitopes on C...

To investigate how bacterial pathogens spread from child to child in a day care center, we monitored six children, two boys and four girls, born between August 1995 and November 1997, attending a day care center and analyzed nasopharyngeal samples from them using pulsed-field gel electrophoresis (PFGE). We obtained nasopharyngeal cultures from all of the affected children and almost all of the ...

IntroductionMoraxella catarrhalis is a gram negative, oxidase positive, cocci mainly causing upper and lowerrespiratory tract infections. The RND family efflux pumps lead to multidrug resistance in most ofthe negative bacteria. One of the well- known M. arcAB oprMsystem. objective this study was investigate antibiotic andto determine its dependence on pump. MethodsIn study, 283 different respir...

The speed and precision of hydrolysis of tributyrin to butyric acid as a test to detect Branhamella catarrhalis were evaluated. The test proved consistently reliable in the identification of strains and correctly differentiated B catarrhalis from Neisseria sp. The combination of Gram stain, oxidase, and catalase tests to tributyrin hydrolysis provides a means of positive same day identification...

The UspA2 protein has been shown to be directly involved in the serum-resistant phenotype of Moraxella catarrhalis. The predicted 5'-untranslated regions (UTR) of the uspA2 genes in several different M. catarrhalis strains were shown to contain various numbers (i.e., 6 to 23) of a heteropolymeric tetranucleotide (AGAT) repeat. Deletion of the AGAT repeats from the uspA2 genes in the serum-resis...

Over 12 months serum was collected from 45 inpatients, with acute bronchopulmonary infection, in whose sputum Branhamella catarrhalis predominated, or was the sole pathogen. Serum was examined for IgG against B catarrhalis using an immunofluorescence antibody test. Acute and convalescent sera were compared with sera of age and sex matched controls. The convalescent sera had significantly higher...

Bioactivities of thiotetrahydropyridines were previously described. Herein, a novel bioactive sulfoxide analog; N-acetyl-2-(1-adamantylsulfoxo)-3-acetoxy-4-phenyl-6-hydroxy-1,2,3,6-tetrahydropyridine (3) from the deoxydative substitution of 4-phenylpyridine 1-oxide is reported. Its structure was elucidated using spectral data including 2D-NMR, MS, IR and UV. The sulfoxide 3 exhibited antibacter...

Otitis media (OM) is a polymicrobial disease wherein prior or concurrent infection with an upper respiratory tract virus plays an essential role, predisposing the middle ear to bacterial invasion. In episodes of acute bacterial OM, respiratory syncytial virus (RSV) is the most commonly isolated virus and thus serves as an important co-pathogen. Of the predominant bacterial agents of OM, the pat...

Secretion of outer membrane vesicles (OMV) is an intriguing phenomenon of Gram-negative bacteria and has been suggested to play a role as virulence factors. The respiratory pathogens Moraxella catarrhalis reside in tonsils adjacent to B cells, and we have previously shown that M. catarrhalis induce a T cell independent B cell response by the immunoglobulin (Ig) D-binding superantigen MID. Here ...