The CBA/N strain of mouse, derived in 1966 from the C B A / H strain (1), carries an X-linked mutation whose expression affects a number of B-cell functions. The B cells in adult CBA/N mice have many of the characteristics of those found in neonatal mice, including a higher density of surface IgM and a lower expression of B-cell differentiation markers, such as Ia, Mls, Lyb3, and Lyb5.1 (2-5). ...

The highly aggressive pediatric sarcomas are characterized by high levels of matrix metalloproteinase (MMP)-2 and MMP-9, which play crucial roles in tumor invasion and metastasis by degradation of the extracellular membrane leading to cancer cell spread to distal organs. We examined the effects of cytokines, mitogens, inducers and inhibitors on MMP-2 and -9 expression in osteosarcoma (U2OS) and...

The prostaglandin (PG) content of mitogen- and antigen-stimulated leukocyte cultures was examined by a radioimmunoassay procedure empolying an antiserum reactive with PGB(1) and PGB(2), the alkaline dehydration products of PGE and PGA. At 48 h, mitogen-activated mouse spleen cell cultures showed 2-10-fold increases in the PGE, but not in the PGA, component of immunoreactive PG (iPG) fractionate...

The CBA/N strain of mouse, derived in 1966 from the C B A / H strain (1), carries an X-linked mutation whose expression affects a number of B-cell functions. The B cells in adult CBA/N mice have many of the characteristics of those found in neonatal mice, including a higher density of surface IgM and a lower expression of B-cell differentiation markers, such as Ia, Mls, Lyb3, and Lyb5.1 (2-5). ...

The antiviral potential of human colostral leucocytes was assessed by their capacity to produce interferon. Leucocytes cultured from colostrum were stimulated by mitogens or Newcastle disease virus (NDV) to produce interferon which, by metabolic and physicochemical criteria, corresponded to normal human leucocyte interferon. Prepartum cells produced higher levels than postpartum cells. Colostra...

Of 52 cancer patients studied for their in vitro response in lymphocyte blastogenesis assays, 50 were also studied for immunocompetence by in vivo assays. The in vivo assays used were the delayed cutaneous hypersensitivity reaction to the primary stimulus of 2,4-dinitrochlorobenzene (DNCB), and the recall reactions to four common microbial antigens. The in vitro assays were the blastogenic resp...

Matrix metalloproteinases (MMPs) secreted by cervical and ovarian cancer, especially MMP-2 and MMP-9, play crucial roles in tumor invasion and metastasis. We examined the effect of cytokines, mitogens, inducers and inhibitors on MMP-2 and MMP-9 expression in cervical and ovarian cancer cell lines. Human cervical (HeLa and DoTc2-4510) and ovarian (SK-OV-3) cell lines were cultured in appropriate...