نتایج جستجو برای: mirna 7
تعداد نتایج: 663954 فیلتر نتایج به سال:
Expression levels of microRNAs (miRNAs) are globally reduced in cancer compared with matched normal tissues, and miRNA function has recently been implicated in tumorigenesis. To test whether epigenetic silencing contributes to miRNA suppression in tumors, lung cancer cells were treated with inhibitors of DNA methylation or histone deacetylation. No significant alteration in miRNA expression was...
Abstract The aberrant expression of microRNA (miRNA) is closely associated with various pathological processes, such as the development gastric cancer. High-efficiency quantification miRNAs significant for diagnosis, prognosis, and treatment cancers. However, sensitive reliable detection miRNA remains a huge challenge. We depict here novel fluorescent approach label-free by exploiting designed ...
As key regulators in cellular functions, microRNAs (miRNAs) themselves need to be tightly controlled. Lin28, a pluripotency factor, was reported to downregulate let-7 miRNA by inducing uridylation of let-7 precursor (pre-let-7). But the enzyme responsible for the uridylation remained unknown. Here we identify a noncanonical poly (A) polymerase, TUTase4 (TUT4), as the uridylyl transferase for pr...
رشد و متابولیسم گیاه تحت تأثیر انواع محرک های زنده و غیرزنده از جمله تنش های محیطی قرار می گیرد که گیاه از طریق هورمون ها به آن ها پاسخ می دهد. mirnaها، گروهی از rnaهای کوچک غیر کدکننده هستند که برخی از آن ها در سیگنالینگ هورمون های گیاهی نقش دارند. در این مطالعه با استفاده از تکنیک qrt-pcr، الگوی بیان mir159a,b، mir160، mir167a,b و mir171a، که به ترتیب در تنظیم بیان فاکتورهای رونویسی myb، arf،...
MicroRNA-146a participates in spinal cord injury (SCI) recovery. Until recently, how miRNA-146a participates in SCI remained unclear. In this study, we tried to explore the roles of miRNA-146a in the recovery of SCI using a rat model. The expression of the probable target genes of miRNA-146a (including IRAK1 and TARF6) as well as proinflammation cytokines were measured until 7 days after surger...
MicroRNAs (miRNA) regulate many genes critical for tumorigenesis. We profiled miRNAs from 11 normal breast tissues, 17 noninvasive, 151 invasive breast carcinomas, and 6 cell lines by in-house–developed barcoded Solexa sequencing. miRNAs were organized in genomic clusters representing promoter-controlled miRNA expression and sequence families representing seed sequence–dependent miRNA target re...
Let-7 microRNA (miRNA) regulates heterochronic genes in developmental timing of the nematode Caenorhabditis elegans. Binding of miRNA to messenger RNA (mRNA) and structural features of the complex are crucial for gene silencing. We herein present the NMR solution structure of a model mimicking the interaction of let-7 miRNA with its complementary site (LCS 2) in the 3' untranslated region (3'-U...
We developed a simple, direct and cost-effective approach to search for the most likely target genes of a known microRNA (miRNA) in vitro. We term this method 'labeled miRNA pull-down (LAMP)' assay system. Briefly, the pre-miRNA is labeled with digoxigenin (DIG), mixed with cell extracts and immunoprecipitated by anti-DIG antiserum. When the DIG-labeled miRNA and bound mRNA complex are obtained...
Whole-mount in situ hybridization (WISH) is an outstanding method to decipher the spatiotemporal expression patterns of microRNAs (miRNAs) and provides important clues for elucidating their functions. The first WISH method for miRNA detection was developed in zebrafish. Although this method was quickly adapted for other vertebrates and fruit flies, WISH analysis has not been successfully used t...
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