The pattern of invasion of human hair in vitro by the dermatophyte Microsporum gypseum was studied by transmission and scanning electron microscopy. Mycelia that invaded the hair cortex through the edge of cuticles showed a flattened "frond" growth in contrast to the filamentous form seen on ordinary laboratory media. The frond cells were characterized by the presence of vesicles formed by inva...

Apama siliquosa Lamk is an important medicinal plant whose leaves and roots have multiple uses. The plant is a rich source of many bioactive compounds which are of medicinal importance. The study was undertaken to investigate the antifungal activity of the methanol, chloroform, ethyl acetate and hot water extracts of the roots of Apama siliquosa. The extracts were tested against the pathogenic ...

Dermatomycoses caused by Microsporum canis are frequent in domestic animals and easily transmissible to humans. Several proteases secreted by this fungus were identified as potential virulence factors, but the construction of deficient strains is required to investigate their role in the pathogenesis of the disease. Using target genes encoding two of these proteases, a first evaluation of the u...

We report the case of a Spanish 60-year-old female who presented in 1997 with onychomycosis of the left thumbnail following an injury caused by a fresh fish bone. Microsporum racemosum was repeatedly cultured from nail scrapings, and its identity was confirmed by sequencing the isolate's ITS1/ITS2 and 5.8S rRNA regions. The patient was successfully treated with itraconazole, which was administe...

Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently reported. Using Aspergillus fumigatus metalloprotease genomic sequence (MEP) as a probe, three genes (MEP1, MEP2, and MEP3) were isolated from an M. canis genomic library. They presented a quite-...

The recurrent evolutionary emergence of asexual lineages within sexual zoo- and anthropophilic dermatophyte species living in animal-frequented soil is likely to be triggered by changes in ecological niche, i.e., shifts of host animal. Subsequent adaptation to the new host species is noted. Sometimes geographic isolation or intrinsic host factors like human race may also play a role in speciati...

A survey of soil from several sites in Franklin and surrounding counties in central Ohio for human-pathogenic fungi was undertaken. The samples were analyzed for the presence of dermatophytes and keratinophytic fungi by the hair-bait technique, for . systemic mycotic agents by a direct spraying method, and for nocardiae by the paraffinbait technique. The hair-bait technique revealed Trichophyto...

Seven new formamido-diterpenes, cavernenes A-D (1-4), kalihinenes E and F (5-6), and kalihipyran C (7), together with five known compounds (8-12), were isolated from the South China Sea sponge Acanthella cavernosa. Structures were established using IR, HRESIMS, 1D and 2D NMR, and single X-ray diffraction techniques. The isolated compounds were assessed for their cytotoxicity against a small pan...

Out of 410 clinically diagnosed cases of superficial mycoses encountered during 1985 - 87, direct KOH mounts were positive in 250 (62.19%) cases and the cultures were positive in 123 (30.00%) cases. The organisms isolated included Trichophyton rubrum (36.58%). T. simii (32.52%), T.mentagrophytes (12.19%), C.tropicum (8.13%), Microsporum gypseum (6.50%), Epidermophyton floccossum (2.43%), T. vio...

background: subtilisin -like proteases are the group of proteases including keratinases found in dermatophytes which de­graded keratin. determination of the proteases activity of trichophyton vanbreuseghemii isolates which were obtained from soil and clinical and soil isolates of microsporum gypseum in iran and characterization of their genome were aim of present study. methods: ezymatic activi...